DNA-InŽ CRISPR

Specifically formulated for CRISPR/Cas9 transfection
DNA-InŽ CRISPR transfection reagent is optimized for large plasmid delivery of CRISPR/Cas9 vectors into a range of cell types. This reagent is especially well-suited for hard-to-transfect primary cells.



Features
  • Optimized for large plasmid DNA containing Cas9/GFP/ guideRNA (all-in-one).
  • High efficiency delivery of Cas9 expression vectors in hard-to-transfect primary cells

Highly efficient Cas9 delivery in primary cells

Human dermal primary fibroblasts (above) were plated to give ~70% plating density on the day of transfection. Cells were transfected with Cas9-GFP 24 hours after plating.  Cells were labelled with anti-GFP (green) to observe low expression of Cas9-GFP vector and Hoechst 3342 (blue) to view nuclei. DNA-InŽ CRISPR transfected cells (left image) show significantly higher Cas9 transfection efficiency vs cells transfected with competitor reagents (L2K, L3K).

Human primary keratinocytes (above) were plated to give a 50-70% plating density on the day of transfection. Cells were transfected with Cas9-GFP 24 hours after plating.  Cells were labelled with anti-GFP (green) to observe low expression of Cas9-GFP vector and Hoechst 3342 (blue) to view nuclei. DNA-InŽ CRISPR transfected cells (left image) show significantly higher Cas9 transfection efficiency vs cells transfected with competitor reagents (L2K, L3K).


C2C12 mouse myoblasts (above) were plated to give a 50-70% plating density on the day of transfection. Cells were transfected with Cas9-GFP 24 hours after plating.  Cells were labelled with anti-GFP (green) to observe low expression of Cas9-GFP vector and Hoechst 3342 (blue) to view nuclei. DNA-InŽ CRISPR transfected cells (left image) show significantly higher Cas9 transfection efficiency vs cells transfected with competitor reagents (L2K, L3K).


Human skeletal muscle cells (above) were plated to give a 50-70% plating density on the day of transfection. Cells were transfected with Cas9-GFP 24 hours after plating.  Cells were labelled with anti-GFP (green) to observe low expression of Cas9-GFP vector and Hoechst 3342 (blue) to view nuclei. DNA-InŽ CRISPR transfected cells (left image) show significantly higher Cas9 transfection efficiency vs cells transfected with competitor reagents (L2K, L3K).

Lipofectamine is a product of Thermo Fisher Scientific Inc.

References
Ran et. al. Nat Protoc. 2013 Nov;8 (11):2281-308. doi: 10.1038/nprot. 2013.143. Epub 2013 Oct 24.