DNA-In« Stem

Optimized for maximum DNA delivery in Stem Cells 
DNA-In« Stem Transfection Reagent offers a simple, robust and reproducible method for delivering DNA into a wide range of stem cells, including neural stem cells. Formulated and optimized specifically for embryonic and adult stem cells, DNA-In« Stem is a new-generation transfection reagent that enables high efficiency transfection while maintaining maximum cell viability and cell growth.

Key Features:
  • Optimized formulation for DNA delivery, including large CRISPR-Cas9 plasmids, into primary and iPS cells.
  • Maximum transfection efficiency and cell viability.
  • Requires only low amounts of DNA for maximum expression and exceptionally low toxicity.
  • Robust, reproducible performance across a wide range of DNA concentrations.

High efficiency CRISPR-Cas9 Delivery 

Neural progenitor cells (HT223-B) from adult brain were transfected with pEF1a-Cas9-2A-GFP plasmid (Addgene) using DNA-In« Stem (left) and Lipofectamine 3000 (right). Data courtesy of Sasha Beylina, Staff Scientist, NIH/NIA
Outperforms competitors across a range of cell types

DNA-In« Stem requires up to 4X less DNA vs competitor reagents for maximum performance.  Human iPSC-derived neural stem cells (NSC), Human embryonic stem cells, adipose-derived Mesencymal Stem Cells and Huntington's Disease (HD)-specific iPSCs plated in 24-well plates were transfected with DNA-In« Stem, Lipofectamine«2000 (L2000) and 3000 (L3000) using various amounts (Ál) of reagent.  Cells transfected with DNA-In« Stem were transfected with both low and high amounts of DNA. Cells were also transfected with L2000 and L3000 using their recommended 500ng of DNA.

Lipofectamine is a product of Thermo Fisher Scientific Inc.