DNA-In« Transfection Reagent

A Universal Transfection Reagent for DNA Delivery
DNA-In« is the newest generation transfection product developed by the lead members of the scientific team that invented the Lipofectamine« and Lipofectamine« 2000. DNA-In« is a novel cationic transfection reagent which balances high transfection and expression levels with low toxicity. DNA-In« 293 is a novel cationic transfection reagent which balances high transfection and expression levels with low toxicity in HEK293 cells.




Features
  • Fast, simple, easy-to-follow protocol
  • One-step process: simply add DNA-In«  Reagent to diluted DNA, incubate 10 minutes, and add to pre-plated cells
  • Minimal toxicity - no need to replace medium after transfection
Superior Transfection Efficiency
Cells were transfected with a plasmid, containing EmeraldGFP behind an EF1α promoter, with either DNA-In« or Lipofectamine«2000, at several different lipid:DNA ratios and examined ~44 hours post-transfection. Representative images of the best conditions for each transfection reagent are shown below:


Low Cell Toxicity


In 293 cells, DNA-In« maintains high cell viability throughout the tested dosage range, while LF2K has a significant cell toxicity issue when used in dosages over 3Ál.

*Lipofectamine is a product of Thermo Fisher Scientific Inc.