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PCR Polymerases
Characteristics of Enzynomics PCR polymerases
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nTaq
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nTaq-HOT
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nTaq-multi
HOT
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nTaq-Tenuto
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nPfu-Forte
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nPfu-Special
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Length
of amplified DNA
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<5
kb
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<5
kb
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<5
kb
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<15
kb
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<15
kb
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<20
kb
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Yield
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+
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+
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+
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+++
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++
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+++
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5'->3'
Exonuclease
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+a
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+a
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+a
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+a
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-
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-
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3'->5'
Exonuclease
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-
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-
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-
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+b
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++
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++
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Error
rate (10-6)
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24
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24
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24
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3.0
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0.28
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0.11
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Ends
of amplified DNA
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A
tail
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A
tail
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A
tail
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A
tail
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Blunt
end
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Blunt
end
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a 5’ to 3’ exonuclease activity is reduced by mutation.
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b 3’ to 5’ proofreading activity of Pfu polymerase is present.
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List of Products..
Standard PCR: nTaq
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nTaq has greatly reduced activity of 5'→3' exonuclease activity. As a result, formation of erroneously amplified products caused by the 5'→3' exonuclease activity of wild type polymerase is effectively prevented |
HOT-Start PCR: nTaq-HOT
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nTaq -HOT is a hot start PCR polymerase which remains inactive at temperatures lower than 75°C as a result of the chemical modifications. Therefore, a heat activation step is required to activate |
High Fidelity PCR: nPfu-Forte
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nPfu-Forte displays higher fidelity than nTaq due to its 3'→5' proofreading exonuclease activity. thus, High-fidelity DNA polymerization is obtained with nPfu-Forte, allowing amplification of DNA up to 10 kb long. |
High Performance PCR: nTaq-Tenuto
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nTaq-Tenuto is nTaq supplemented with 3'→5’ proofreading activity and a PCR enhancing factor for improved efficiency and fidelity. nTaq-Tenuto can be used to amplify DNA longer than 10 kb, which is difficult with common Taq polymerases alone. Thus, this product is improved in both fidelity (> 2 fold) of PCR products and amplification efficiency of longer PCR products. |
Multiplex PCR: nTaq-multi HOT
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nTaq-multi HOT is a hot start PCR polymerase which remains inactive at temperatures lower than 75°C Therefore, a heat activation step is required to activate nTaq-multi HOT. nTaq-multi HOT produces up to 20 different amplified products with a single tube reaction. This product can be used in general multiplex PCR and genotyping experiment related to genetic diagnostics. |
Clean PCR: nTaq-Pure
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nTaq-Pure is a highly purified polymerase especially for PCR reactions where freedom from bacterial genomic DNA is essential. nTaq-Pure was purified by state of the art technology for minimizing the E. coli genomic DNA contamination. |
Prevent carryover contamination PCR
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Uracil-DNA Glycosylase (UDG) catalyses the release of free uracil from uracil-containing DNA. UDG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA. Thus UDG plus PCR polymerase eliminate uracil-product amplicon carry-over. |
Others
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DNA Polymerases |
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RNA Polymerases |
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