Long Taq mix (x2)
#EZ-2061

Contents:2X Hy- LongTaq Mix      1ml
Nuclease-free water1ml
PCR Enhancer0.5ml

Store at -20C

Description
Long Taq mix (x2) is a premixed, ready-to-use solution containing Long Taq DNA Polymerase, dNTPs, MgCl2 and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only primers and template DNA are added. Long Taq Mix contributes to highly reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination. It also contributes to higher sensitivity by adding enhancer. Long taq DNA Polymerase, a combination of two thermostable DNA polymerases, Taq and High Fidelity, is a special formulation designed for amplifying large fragment. This specially formulated Long Taq was shown to amplifiy long templates from λ phage genome of up to 20 kb. It is also a better choice for amplifying complex template, such as GC-rich template. Long taq is suitable as a direct replacement for ordinary Taq Polymerase in most applications. Use of Long Taq in your PCR reactions results in 3-dA overhangs PCR products, which can be used in TA clone.

Features
  • Convenient: only primers and template DNA are added when prepare final PCR. 
  • High efficiency: saving your time by simplifying the process 
  • Reproducible: reducing the risk of pipetting errors, miscalculation and contamination. 
  • Longer fragment: amplify long templates as long as 40kb. 
  • Amplification of complex template (GC rich or repetitive sequence
Applications
  • PCR for long templates of up to 40kb. 
  • High reproducible, high throughput PCR for complex template.
Composition of the Long Taq Mix
0.25U/ul Taq DNA polymerase, 2X PCR buffer, 0.4mM dNTPs, 3.2 mM MgCl2, 0.02% bromophenol blue. Long Taq mix buffer is a proprietary formulation optimized for robust performance in PCR.