PCR Ready Mix (x2)
#EZ-3006   1ml, 100rxn

Contents:PCR Ready Mix      1ml
Nuclease-free water1ml

Store at -20C
Shelf life: 2 years


Description
PCR Ready Mix (2x) is a premixed, ready-to-use solution containing Taq DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only primers and template DNA are added. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set up. The mix retains all features of Taq DNA Polymerase. Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. Taq DNA Polymerase can amplify DNA target up to 5 kb (simple template). The elongation velocity is 0.9~1.2kb/min (70~75C). It has 5' to 3' polymerase activity but lacks of 3' to 5' exonuclease activity that results in a 3'-dA overhangs PCR product.

Features
  • Convenient–Taq DNA Polymerase in a ready-to-use mixture.
  • High yields of PCR products with minimal optimization.
  • Fast -saves time due to reduced number of pipetting steps.
  • Reproducible -lower contamination and pipetting error risk

Applications
  • High throughput PCR.
  • Routine PCR with high reproducibility
  • Generation of PCR products for TA cloning
Composition of the 2x High Fidelity Ready Mix
Taq DNA polymerase is supplied in 2X Taq buffer, dNTPs, 3mM MgSO4 and bromophenol blue. Taq Ready mix buffer is a proprietary formulation optimized for robust performance in PCR.

Perform PCR using the following thermal cycling conditions:
Initial Denaturation94C3 minutes
25-35 Cycles 94C
55-68C
72C
30 seconds
30 seconds
1 min
Final Extension72C10 minutes