Hy-RNase A
Cat No. N9046
Size: 100mg

Contents: Hy-RNase A: 100mg
Specific Activity: >2500 u/mg protein (>50 Kunitz units/mg protein)
Form: Contains 100 mg Hy-RNase A, Lyophilized Powder


Description
Hy-RNase A, DNase and protease-free is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. It cleaves the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide, resulting 2', 3'-cyclic phosphate hydrolyzed to the corresponding 3'-nucleoside phosphate.

Features
Hy-RNase A is free of DNase activity. It is not necessary to heat it before use.

Applications
-Plasmid and genomic DNA preparation
-Removal of RNA from recombinant protein preparations.
-Ribonuclease protection assays
-Mapping single-base mutations in DNA or RNA

Definition of Activity Unit

One unit of the enzyme causes an increase in absorbance of 1.0 at 260 nm when yeast Hy-RNA is hydrolyzed at 37C and pH 5.0.
Fifty units are approximately equivalent to 1 Kunitz unit
.

Storage
Stable at room temperature for one year. For long time storage, store the Hy-RNase A powder at 4C.

Source
Bovine pancreas.

Molecular Weight
13.7 kDa monomer.

Quality Control
The absence of endodeoxyribonucleases, exodeoxyribonucleases and proteases confirmed by appropriate quality tests, functionally tested for RNA digestion in a plasmid DNA purification procedure.