Real-time PCR Reagents

List of Products..

Real-time PCR (SYBR Green Chemistry)

TOPreal™ qPCR 2X PreMIX (SYBR Green) is a preassembled liquid mixture that contains nTaq-HOT, optimal reaction buffer, dNTP, stabilizingagents, ROX reference dye and SYBR Green I dye. TOPreal™ qPCR 2X PreMIX (SYBR Green) is formulated to have enhanced specificity, thereby minimizing nonspecific noise signal due to formation of primer dimers or non-specific products

Real-time PCR (Probe)

TOPreal™ qPCR 2XPreMIX (Probe) provides accurate real-time quantification of DNA and cDNA targets (100~150 bp in length). High specificity and sensitivity in PCR are achieved by the use of the hot-start enzyme together with a specialized PCR buffer. TOPreal™ qPCR 2XPreMIX (Probe) is a liquid mixture that contains Taq Polymerase (for hot start PCR), optimal reaction buffer, dNTP, stabilizing agents

One-step RT qPCR Kit

TOPreal™ One-step RT qPCR Kit (SYBR Green) is a ready-to-use solution optimized for Real-Time One Step RT-PCR including SYBR Green I and ROX as a reference dye. The easy one-tube setup minimizes the risk of contamination. This Kit contains nTaq-HOT DNA polymerase, which prevents extension of nonspecifically annealed primers and primer–dimers, and TOPscript™ II Reverse Transcriptase (RNase H-), high yield of synthesizing cDNA. The optimized components result in highly sensitive and successful results.





   *SYBR is a trademark of Molecular Probes Inc.