NutriStem® hESC XF

   
    
    
Product

Cat.#

Size

Storage Temp
AF NutriStem® hESC XF, without HSA 05-102-1A 500ml -20ºC
AF NutriStem® hESC XF, without HSA 05-102-1B 100ml -20ºC
    
    


Instructions for use Technical manual Brochure

Defined, xeno-free, serum-free, albumin-free medium, designed to support the growth of human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC)

AF NutriStem® hESC XF contains only the most essential components required for maintenance of ES cells and iPS cells, providing a simpler medium for pluripotent stem cell culture.

AF NutriStem® hESC XF was launched in early 2009 and was developed and validated in cooperation with Prof. Joseph Itskovitz-Eldor M.D., D.Sc from the, Technion-Israel Institute of Technology.

A paper in the ".May 2011 issue of Nature Methods by James Thomson at the University of Wisconsin describes a stem cell culture medium, E8, that has been stripped of all unnecessary components."(1) AF NutriStem® hESC XF contains no albumin, and was originally pared of other ingredients that Thomson and co-workers just recently deemed to be unnecessary.

Human ES cells (i.e., H9.2, I6, I3.2, H1) cultured in NutriStem® media displayed:

  • The ability to self-renew, by expansion in feeder-free culture conditions (Matrigel), on human-feeder layer (HFF) or on Mouse feeder cells (MEFs);
  • Expression of hESC specific transcription factors and antigens
  • Pluripotency by the ability to differentiate into the three germ layers (endoderm, ectoderm and mesoderm) in-vitro by EB formation and in-vivo by teratoma formation in SCID mice; and
  • Human ES cell normal phenotype and genotype

NutriStem® human ES & iPS cell culture media provides:

  • Consistent media performance and predictable cellular behavior derived from a defined xeno-free formulation
  • Increased reproducibility shown in long-term growth of over 50 passages
  • Cost efficiency obtained in low human recombinant growth factor concentrations (rh bFGF, rh TGFß)

Featurs:

  • Albumin-Free
  • Optimized for feeder-dependent culture (i.e. HFF and MEF)
  • Superior performance on HFF feeder layer
  • Ready-to-use
  • When culturing on feeder-free layer (i.e. Matrigel) an addition of 5% of Bio-Pure Human Serum Albumin (HSA solution, 10%) (Cat.# 05-720-1) is recommended.

Storage
Store at -20ºC

Stability
18 months

Quality Control
NutriStem® is routinely tested for optimal maintenance and expansion of undifferentiated hESCs. Additional standard evalutions are pH, osmolality, endotoxins and sterility tests.

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