TOPscript™ II Reverse Transcriptase (RNase H-)

TOPscript™ II Reverse Transcriptase (RNase H-) is genetically engineered recombinant protein that possesses moderate thermostability (42~55°C) and lacks RNase H activity. TOPscript™ II Reverse Transcriptase (RNase H-) does not degrade RNA strands from RNA-DNA hybrids and therefore is capable of high yield of synthesizing cDNA. The optimum temperature of TOPscript™ II Reverse Transcriptase (RNase H-) is 50°C. TOPscript™ II Reverse Transcriptase (RNase H-) does not show inhibitory effect on one-step RT-PCR reaction.