TOPscript™ III Reverse Transcriptase (High fidelity) TOPscript™ III Reverse Transcriptase (High fidelity) is a blend of a recombinant reverse transcriptase and a proofreading enzyme. The synergistic effect of both enzymes generate 7-fold higher fidelity compared to other commonly used reverse transcriptases. Due to the higher thermostability of reverse transcriptase and the specially optimized buffer system, reverse transcription reaction is possible at temperatures up to +55°C. TOPscript™ III Reverse Transcriptase (High fidelity) does not degrade RNA strands from RNA-DNA hybrids and therefore is capable of high yield of synthesizing cDNA. The optimum temperature of TOPscript™ III Reverse Transcriptase (High fidelity) is 50°C. TOPscript™ III Reverse Transcriptase (High fidelity) does not show inhibitory effect on one-step RT-PCR reaction.
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