Taq + dNTPs
#EZ1012, 500U

Concentration: 5U/μl

Contents:Hy-Taq DNA polymerase100μl
10xPCR Buffer (Mg2+ Plus)1.25ml
dNTPs (2.5mM each)1ml
6xLoading Buffer1ml

Store at -20C

Description
Taq 500U + dNTPs is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. Taq DNA Polymerase can amplify DNA target up to 5 kb (simple template). The elongation velocity is 0.9~1.2kb/min (70~75C). It has 5' to 3' polymerase activity but lacks of 3' to 5' exonuclease activity that results in a 3'-dA overhangs PCR product.

Unit Definition
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmole of dNTPs into an acid-insoluble form in 30 minutes at 70C using hering sperm DNA as substrate.

Storage Buffer
20mM TrisCl ( pH8.0), 100mM KCl, 3.2mM MgCl2, 1mM DTT, 0.1% Triton X-100, 0.1% Tween20, 0.2mg/ml BSA, 50% (v/v) glycerol.

10X PCR Buffer with Mg2+
100mM Tris-HCl (PH 8.8), 500mMKCl, 1%Triton-X-100, 16mM MgCl2.

Applications
  • PCR amplification of DNA fragments as long as 5 kb
  • DNA labeling
  • DNA sequencing
  • PCR for cloning