Multiplex QF-PCR Assay for
Rapid Testing of Y Chromosome Microdeletion


Product Overview:

  • Consists of 14 primer pairs that are homologous to previously identified & mapped sequence-tagged sites (STSs)
  • Out of 14, 8 markers (Sy84, Sy86, Sy127, Sy134, Sy254, Sy255, SRY and ZFY) are determined by European Molecular Genetics Quality Network guidelines and the remaining 6 are markers for all regions.
  • Five-dye fluorescent system for automated DNA fragment analysis for multiplex amplification & electrophoresis

Main Features:
  • Relative quantification between Chr.X & Chr.Y
  • Precise & quick detection for all STSs in the same plot screen
  • Coverage of the maximum number of possible Y chromosome microdeletion
  • SRY is a control marker for the testis determining factor on the short arm of the  chromosome and allows XX males arising from Y to X translocations to be detected
  • Extensively tested for reproducibility and robustness, and quality controlled to ensure optimum performance

STS included in the Y Chromosome Microdeletion Detection System

 

Marker

Region

BP

Dye

SRY(Y14)

Control

464

VIC

ZFY

Control

491

VIC

Sy255

AZFc

123

VIC

Sy134

AZFb

303

VIC

RBMY

RBMY

238

6-FAM

AZFa

AZFa(Prox2)

217

6-FAM

Sy133

AZFb

176

6-FAM

Sy152

AZFd

124

PET

Sy153

AZFd

136

6-FAM

Sy157

AZFc

291

PET

Sy84

AZFa

330

PET

Sy86

AZFa

317

NED

Sy127

AZFb

271

NED

Sy254

AZFc

382

PET

 




Ordering Information

Pack Size Catalog No.
25 tests206025
50 tests206050
100 tests206100