Multiplex
QF-PCR Assay for Rapid Testing of Y Chromosome Microdeletion
Product Overview:
- Consists of 14 primer pairs that are homologous to previously identified & mapped sequence-tagged sites (STSs)
- Out of 14, 8 markers (Sy84, Sy86, Sy127, Sy134, Sy254, Sy255, SRY and ZFY) are determined by European Molecular Genetics Quality Network guidelines and the remaining 6 are markers for all regions.
- Five-dye fluorescent system for automated DNA fragment analysis for multiplex amplification & electrophoresis
Main Features:
- Relative quantification between Chr.X & Chr.Y
- Precise & quick detection for all STSs in the same plot screen
- Coverage of the maximum number of possible Y chromosome microdeletion
- SRY is a control marker for the testis determining factor on the short arm of the chromosome and allows XX males arising from Y to X translocations to be detected
- Extensively tested for reproducibility and robustness, and quality controlled to ensure optimum performance
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STS included in the Y Chromosome Microdeletion Detection System
Marker
|
Region
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BP
|
Dye
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SRY(Y14)
|
Control
|
464
|
VIC
|
ZFY
|
Control
|
491
|
VIC
|
Sy255
|
AZFc
|
123
|
VIC
|
Sy134
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AZFb
|
303
|
VIC
|
RBMY
|
RBMY
|
238
|
6-FAM
|
AZFa
|
AZFa(Prox2)
|
217
|
6-FAM
|
Sy133
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AZFb
|
176
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6-FAM
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Sy152
|
AZFd
|
124
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PET
|
Sy153
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AZFd
|
136
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6-FAM
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Sy157
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AZFc
|
291
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PET
|
Sy84
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AZFa
|
330
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PET
|
Sy86
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AZFa
|
317
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NED
|
Sy127
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AZFb
|
271
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NED
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Sy254
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AZFc
|
382
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PET
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Ordering Information
Pack Size |
Catalog No. |
25 tests | 206025 |
50 tests | 206050 |
100 tests | 206100 |
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