ATZ Labs - a subsidiary of Life Technologies (India) Pvt. Ltd.
Life Technologies India
PhytoTechnology Laboratories

Bacitracin Zinc
Product ID: B132

Introduction

Description: Bacitracin Zinc interferes with cell well biosynthesis in gram-positive bacteria.
USP Grade.
See product info sheet for more information.

Solubility Water at 5.1 mg/mL (28 C) per
The Merck Index; also soluble in DMSO at 1 mg/mL and Ethanol at 2 mg/mL
Physical Form Solid
Storage Temp. 2 to 6 C
UPC / SKU B132
CAS NUMBER 1405-87-4
Formula Weight 1486.07
Formula C66H101N17O16SZn
Storage Temp. 2 to 6 C
Tariff Code 2941.90.1050
Risk Info (R) 20/21/22
Safety Info (S) 22-24/25-36/37/39
No information available

B132 Bacitracin Zinc

Synonym: N/A
CAS: 1405-89-6
Formula: C66H101N17O16SZn
Molecular Wt: 1486.07
Properties
Form: Powder
Appearance: Cream to Tan Powder
Application: Plant Tissue Culture Antibiotic
Solubility: Soluble in Water:5.1 mg/mL (28 C), methanol at 6.55 mg/mL, ethanol at2.0 mg/mL, and isopropanol at 0.16 mg/mL per the Merck Index. PhytoTechnology Labs tests solubility at 1.0 mg/mL in DMSO.
Storage Temp: 2 to 6C
Storage Temp of Stock Solution: The preparation of stock solutions is not recommended. Bacitracin has been observed to lose potency in solution (Merck Index). For optimal potency solutions should be prepared fresh.
Other Notes: Minimum Potency: 65 IU/mg (see Certificate of Analysis for lot specificactivity)

Application Notes
Bacitracin interferes with cell wall biosynthesis in Gram -positive bacteria by inhibiting the dephosphorylation of lipid pyrophosphate . It is also active in some Gram -negative organisms although most are resistant.2

Bacitracin Zn is a mixture of bacitracin A, B1, B2, B3, and Zn. It has been reported that the bacteriostatic activity of bacitracin increases by twofold when Z n was added at 1 x 10 -5 M and 10- fold at 1 x 10 -4 M. Additionally, the presence of Zn ion helps enhance thermal and storage stability of the bacitracin.3

It has been reported that bacitracin's minimum inhibitory concentration for Clostridium perfringens is 0.93 g/mL . 4 Please Note: It is the sole responsibility of the purchaser to determine the appropriateness of this product for the specific plants that are being cultured and applications that are being used.

References
1. Merck 13, 938
2. Martindale: The Complete D rug Reference , 3 5th ed., Paul S. Blake , Ed. (Royal Pharmaceutical Society, 2007), p. 187.
3. Smith, J.L., and E.D. Weinberg. 1962. Mechanisms of Antibacterial Action of Bacitracin. J. Gen. Microbiol. 28:559- 569.
4. Richez P., Richard A., Cornez B. and Vancraeynest D. "Susceptibility, resistance and antibiotic profile of bacitracin against clostridium perfringens strains isolated during clinical outbreaks of epizootic rabbit enteropathy." 9th World Rabbit Congress - June 10- 13, 20 08 - Verona, Italy.