ATZ Labs - a subsidiary of Life Technologies (India) Pvt. Ltd.
Life Technologies India
PhytoTechnology Laboratories

Bold's Basal Medium (BBM) Solution 50x
Product ID: B1650

Introduction

Description: Bold's Basal Medium (BBM) is a freshwater algae medium that has been used to grow a variety of green algal cultures (e.g. TrichosarcinaChlorococcum, and Chlorella) without the need for soil-extract or vitamins (Brown et al., 1964; Nichols and Bold, 1965). The predominantly inorganic nature of this medium facilitates itself as an axenic-culture maintenance medium (Nichols and Bold, 1965). Solution at a 50x concentration.

Requires 1 mL/L Sulfuric Acid, 0.1% (v/v) (Product # S7664).

Solubility Water
Physical Form Solid
Powder Form B1675
Concentration 50x
Sterility Sterile Filtered
Storage Temp. 2-6 °C
Other Notes Use at 20.0 mL/L for a 1X concentration
UPC / SKU B1650
Storage Temp. 2-6 C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
B210 Boric Acid 571
C266 Calcium Chloride, Anhydrous 943.6
C193 Cobalt Nitrate, Hexahydrate 24.5
C375 Cupric Sulfate, Pentahydrate 78.5
E410 EDTA, Disodium Salt 3180.5
F263 Ferrous Sulfate, Heptahydrate 249
M455 Manganese Chloride, Tetrahydrate 72
P672 Potassium Hydroxide 1550
P705 Potassium Phosphate, Dibasic 3750
P846 Potassium Phosphate, Monobasic, Anhydrous 8750
S624 Sodium Chloride 1250
M651 Sodium Molybdate (VI), Dihydrate 59.7
S802 Sodium Nitrate 12500
Z101 Zinc Sulfate, Heptahydrate 441

B1650 Bold's Basal Medium, 50x

Synonyms: BBM
Properties
Form: Liquid
Appearance: Clear with Light Green to Purple Tint
Application: Freshwater algal culture
Solubility: Miscible with Water
Typical Working Concentration: 20.0 mL/L
Storage Temp: 2-6C
Other Notes: Approx. Solution pH is 4.2 +/- 0.5
It is suggested not to autoclave this product in its 50x concentrate form, as precipitation may result. Product may develop a purple tint over time.
Formula (mg/L)
Boric Acid 571
Calcium Chloride, Anhydrous 943.6
Cobalt Nitrate•6H2O 24.5
Cupric Sulfate•5H2O 78.5
EDTA, Disodium Salt 3180.5
Ferrous Sulfate•7H2O 249
Magnesium Sulfate, Anhydrous 1831.3
Manganese Chloride•4H2O 72
Sodium Molybdate 59.7
Potassium Hydroxide 1550
Potassium Phosphate, Dibasic 3750
Potassium Phosphate, Monobasic 8750
Sodium Chloride 1250
Sodium Nitrate 12500
Zinc Sulfate.7H2O 441

Application Notes
Bold's Basal Medium (BBM) is a freshwater algae medium that has been used to grow a variety of green algal cultures (e.g. Trichosarcina, Chlorococcum, and Chlorella) without the need for soil-extract or vitamins (Brown et al., 1964; Nichols and Bold, 1965). The predominantly inorganic nature of this medium facilitates itself as an axenic-culture maintenance medium (Nichols and Bold, 1965).

Other algae species commonly grown in Bold's Basal Medium:
Zyngogonium ericetorum (Stancheva et al., 2014)

Media Preparation:
The standard medium is prepared as follows: Add 20.0 mL of the BBM 50x concentrate to ~0.98 liters of DI water. The final solution pH is adjusted to 6.6 +/- 0.1 with KOH (Stein, 1973).

References
Brown, R.M., Larson, D.A., and H.C. Bold. (1964) Science 143(3606), 583-585.
Nichols H.W., and H.C. Bold (1965) J. Phycology 1, 34-38.
Stein J. (1973) Handbook of Phycological methods. Culture Methods and Growth Measurements. Cambridge University Press. 448 pp