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Bold's Basal Medium (BBM)
Product ID: B1675

Introduction

Description: Bold's Basal Medium (BBM) is a freshwater algae medium that has been used to grow a variety of green algal cultures (e.g. TrichosarcinaChlorococcum, and Chlorella) without the need for soil-extract or vitamins (Brown et al., 1964; Nichols and Bold, 1965). The predominantly inorganic nature of this medium facilitates itself as an axenic-culture maintenance medium (Nichols and Bold, 1965).

Requires 1 mL/L Sulfuric Acid, 0.1% (v/v) (Product # S7664).

Solubility Water
Use With S7664
Physical Form Solid
Solutions Available B1411 (50x)
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 0.705
Other Notes Add 1.0 mL of 0.1% Sulfuric Acid (S7664) to complete this medium.
UPC / SKU B1675
Storage Temp. 2-6 C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
B210 Boric Acid 11.42
C266 Calcium Chloride, Anhydrous 18.87
C193 Cobalt Nitrate, Hexahydrate 0.49
C375 Cupric Sulfate, Pentahydrate 1.57
E410 EDTA, Disodium Salt 63.61
F263 Ferrous Sulfate, Heptahydrate 4.98
M150 Magnesium Sulfate, Anhydrous 36.63
M455 Manganese Chloride, Tetrahydrate 1.44
P672 Potassium Hydroxide 31
P705 Potassium Phosphate, Dibasic 75
P846 Potassium Phosphate, Monobasic, Anhydrous 175
S624 Sodium Chloride 25
M651 Sodium Molybdate (VI), Dihydrate 1.19
S802 Sodium Nitrate 250
Z101 Zinc Sulfate, Heptahydrate 8.82

B1675 Bold's Basal Medium

Synonyms: BBM
Properties
Form: Powder
Appearance: Off-White to Gray/Green
Application: Freshwater algal culture
Solubility: Soluble in Water
Typical Working Concentration: 0.705 g/L
Storage Temp: 2-6C
Storage Temp of Stock Solution: 2-6C
Other Notes: In order to conform with the original reference, 1.0 mL of 0.1% Sulfuric Acid Solution should be added per liter of this medium when prepared at a 1x concentration.
Product or solutions may develop a purple tint over time.
Formula (mg/L)
Boric Acid 11.42
Manganese Chloride.4H2O 1.44
Calcium Chloride, Anhydrous 18.87
Potassium Hydroxide 31
Cobalt Nitrate.6H2O 0.49
Potassium Phosphate, Dibasic 75
Cupric Sulfate.5H2O 1.57
Potassium Phosphate, Monobasic 175
EDTA, Disodium Salt 63.61
Sodium Chloride 25
Ferrous Sulfate.7H2O 4.98
Sodium Molybdate 1.19
Magnesium Sulfate, Anhydrous 36.63
Sodium Nitrate 250
Zinc Sulfate.7H2O 8.82

Application Notes
Bold's Basal Medium (BBM) is a freshwater algae medium that has been used to grow a variety of green algal cultures (e.g. Trichosarcina, Chlorococcum, and Chlorella) without the need for soil-extract or vitamins (Brown et al., 1964; Nichols and Bold, 1965). The predominantly inorganic nature of this medium facilitates itself as an axenic-culture maintenance medium (Nichols and Bold, 1965).

Other algae species commonly grown in Bold's Basal Medium:
Zyngogonium ericetorum (Stancheva et al., 2014)

Media Preparation:
The standard medium is prepared as follows: Add 1.0 mL of 0.1% Sulfuric Acid solution to 1 liter of DI water along with 0.705 g of B1675. The final solution pH is adjusted to 6.6 +/- 0.1 with KOH (Stein, 1973).

References
Brown, R.M., Larson, D.A., and H.C. Bold. (1964) Science 143(3606), 583-585.
Nichols H.W., and H.C. Bold (1965) J. Phycology 1, 34-38.
Stancheva, R., Hall, J. D., Herburger, K., Lewis, L. A., McCourt, R. M., Sheath, R. G., & Holzinger, A. (2014).
Phylogenetic position of Zygogonium ericetorum (Zygnematophyceae, Charophyta) from a high alpine habitat and ultrastructural characterization of unusual aplanospores. Journal of Phycology, 50(5), 790-803.
Stein J. (1973) Handbook of Phycological methods. Culture Methods and Growth Measurements. Cambridge University Press. 448 pp.