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Blaydes Basal Medium
Product ID: B514

Introduction

Solubility Water
Physical Form Solid
Storage Temp. 2-6 C
Grams of powder to prepare 1 Liter 31.86
Other Notes Plant Tissue Culture Tested
UPC / SKU B514
Storage Temp. 2-6 C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
A300 Ammonium Nitrate 1000
B210 Boric Acid 1.6
C180 Calcium Nitrate 241.1
E410 EDTA, Disodium Salt 74.5
F263 Ferrous Sulfate, Heptahydrate 55.7
M150 Magnesium Sulfate, Anhydrous 17.1
M250 Manganese Sulfate 4.4
P704 Potassium Chloride 100
P840 Potassium Iodide 0.8
P100 Potassium Nitrate 65
P846 Potassium Phosphate, Monobasic, Anhydrous 300
Z101 Zinc Sulfate, Heptahydrate 1.5
G503 Glycine 2.0
S391 D-Sucrose 30,000
T390 Thiamine HCl 0.1

B514 Blaydes Modified Basal Medium

Properties
Form: Powder
Appearance: Off-white to yellow
Application: Plant Tissue Culture
Solubility: Soluble in Water
Typical Working Concentration: 31.86 g/L
Storage Temp: 2-6°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients, sucrose, and thiamine as described by Blaydes (1966). Plant Tissue Culture Tested.
Formula (mg/L)
Ammonium Nitrate 1000
Boric Acid 1.6
Calcium Nitrate 241.1
Na2EDTA.2H2O 74.5
Ferrous Sulfate.7H2O 55.7
Magnesium Sulfate, Anhydrous 17.1
Manganese Sulfate.H2O 4.4
Potassium Chloride 65
Potassium Iodide 0.8
Potassium Nitrate 100
Potassium Phosphate, Monobasic 300
Zinc Sulfate.7H2O 1.5
Glycine (Free Base) 2
Sucrose 30,000
Thiamine.HCl 0.1

Application Notes
Plant species: Alfalfa
Callus initiation was achieved when the basal medium was supplemented with 1.9 mg/L Kinetin and 8.0 – 19.0 mg/L 2,4-D. Regeneration was achieved when the Kinetin and 2,4-D were omitted and the basal medium was supplemented with 100 mg/L myo-Inositol and 2,000 mg/L yeast extract

References
Blaydes, OF. 1966. Interaction of kinetin and various inhibitors in the growth of soybean tissue. Physiol. Plant 19: 748-753.