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PhytoTechnology Laboratories

Cefotaxime Solution (250 mg/mL)
Product ID: C1880

Introduction

Description: Cefotaxime is a 3rd generation cephalosporin and it is highly effective against gram-negative bacteria. Cefotaxime is often used in transformation of plant species for the elimination of Agrobacterium tumefaciens.
See product info sheet for more information.

Miscibility Water
Physical Form Liquid
Concentration 250 mg/mL
Sterility Sterile Filtered
Storage Temp. -20 to 0 C
UPC / SKU C1880
CAS NUMBER 64485-93-4
Formula Weight 477.4
Formula C16H16N5O7S2Na
Synonyms (6R,7R)-3-[(Acetyloxy)methyl]-7- [[(2Z)-(2-amino-4-thiazolyl) (methoxyimino)acetyl]amino] -8-oxo-5-thia-1-azabicyclo [4.2.0]oct-2-ene-2-carboxylic Acid
Manufactured from C380
Additional Charge Dry Ice ($15)
Storage Temp. -20 to 0 C
Tariff Code 2941.90.1010
Risk Info (R) 42/43-36
Safety Info (S) 26-36/37/39
No information available

C1880 Cefotaxime Solution 250 mg/mL

Synonym: (6R,7R)-3-[(Acetyloxy)methyl]-7-[[(2Z)-(2- amino-4-thiazolyl)(methoxyimino)acetyl] amino]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct- 2-ene-2-carboxylic Acid, Sodium Salt
CAS: 64485-93-4
Formula: C16H16N5O7S2Na
Molecular Wt: 477.4
Properties
Form: Liquid
Appearance: Light Yellow
Application: Plant Tissue Culture Antibiotic
Solubility: Miscible with Water
Storage Temp: -20 to 0 o C
Typical Working Concentration: 50 to 250 mg/mL
Storage Temp of Stock Solution: Aqueous solution at a pH 4.5-6.2 is stable for 7-14 days when stored at 2 to 6°C. Recommended long term storage at -20°C (i.e., non-frost freezer)

Application Notes
Cefotaxime is an inhibitor of cell wall synthesis and chemically related to penicillin. It is highly effective against Gram-negative bacteria1 . Cefotaxime is often used in transformation research for the elimination of Agrobacterium tumefaciens. Additionally, cefotaxime also has stimulatory effect. It is founded that addition of cefotaxime to medium enhances shoot organogenesis in vitro for many plants (apple, barley, grain, maize, etc.).2,3

A concentration of 90 µg/mL is recommended to achieve microbe toxicity. Cefotaxime concentrations should not exceed 100 µg/mL in order to avoid toxicity for plants (though plant toxicity may be higher or lower than 100 µg/mL for different plant species).

Please Note: While PhytoTechnology Laboratories® tests each lot of this product with two or more plant cell/ tissue culture lines, it is the sole responsibility of the purchaser to determine the appropriateness of this product for the specific plants that are being cultured and applications that are being used.

References
1. Merck 13, 1946
2. Danilova, S.A., Yu. I. Dolgikh. 2004. The stimulatory effect of the antibiotic cefotaxime on plant regeneration in maize tissue culture. Russian Journal of Plant Physiology. 51. pp 559 -562.
3. Rao, A.m., K. Padma Sree, and P.B. 1995. Kavi Kishor. Enhanced plant regeneration in grain and sweet sorghum by asparagines, proline and cefotaxime. Plant Cell Reports. 15. pp 72-75.