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24-Epibrassinolide
Product ID: E244

Introduction

Description:
24-Epibrassinolide has been shown to elongate plant cells through down regulation of the transcriptional repressorIBH1 in Arabidopsis, (22R/S,23R/S,24R)-2α,3α,22,23-Tetrahydroxy-β-homo-7-oxa-5α-ergostan-6-one

Solubility DMSO, or Water at 5 mg per Liter
Physical Form Solid
Storage Temp. -20 to 0 °C
Other Notes Plant Tissue Culture Tested
UPC / SKU E244
CAS NUMBER 78821-43-9
Formula Weight 480.68
Formula C28H48O6
Synonyms (22R/S,23R/S,24R)-2α,3α,22,23-Tetrahydroxy-β-homo-7-oxa-5α-ergostan-6-one
Storage Temp. -20 to 0 C
Tariff Code 2937.29.9090
Risk Info (R) 22-36/37/38
Safety Info (S) 26-36
No information available

E244 24-Epibrassinolide

Synonyms: (22R/S,23R/S,24R)-2α,3α,22,23-Tetrahydroxy-β-homo-7-oxa-5α-ergostan- 6-one
CAS: 78821-43-9
Formula: C27H48O6
Mol. Weight: 480.68
Properties
Form: Crystalline Powder
Appearance: White, Fine to Coarse Powder
Purity: >90%
Application: Plant Growth Regulator
Solubility: DMSO at 1 mg/mL
Typical Working Concentration: N/A
Storage Temp: 20ºC
Other Notes: Plant Tissue Culture Tested

Application Notes
This molecule has been shown to increase plant cell elongation. Both gibberillins and brassinosteroids are known to positively regulate this elongation through over expression of Arabidopsis thaliana transcription factor PRE1 (PACLOBUTRAZOL-RESISTANT1) (Zhang et al. 2009). PRE1 is also an antagonist of the transcriptional repressor IBH1 (ILI1 BINDING BHLH PROTEIN1) which inhibits this elongation (Ikeda et al. 2012). eBL is an active form of brassinosteroids and it also down regulates the expression of IBH1.

References
Bushra Qayyum, Muhammad Shahbaz and Nudrat Aisha Akram. “Interactive Effect of Foliar
Application of 24-Epibrassinolide and Root Zone Salinity on Morpho-physiological Attributes of Wheat (Triticum aestivum L.)” Int. J. Agri. Biol., Vol. 9, No. 4, 2007
Bajguz A, and Asami T. “Suppression of Wolffia arrhiza growth by brassinazole, an inhibitor of brassinosteroid biosynthesis and its restoration by endogenous 24-epibrassinolide.” Phytochemistry 2005 Aug;66(15):1787-96. Dhaubhadel S, Chaudhary S, Dobinson KF and Krishna P. “Treatment with 24-epibrassinolide, a brassinosteroid, increases the basic thermotolerance of Brassica napus and tomato seedlings.” Plant Mol Biol. 1999 May;40(2):333-42. František Hnilicka, Helena Hnilicková, Jaroslava Martinkova, Ladislav Bláha and Pavel Kadlec. “Impact of 24-Epibrassinolide on Chemical Structure and Energy Content in Wheat Grain.” ISSN 1648-116X LŽUU MOKSLO DARBAI. 2009. Nr. 83 (36).
František Franěka, Tomáš Eckschlagerb and Ladislav Kohoutc. “24-Epibrassinolide at Subnanomolar Concentrations Modulates Growth and Production Characteristics of a Mouse Hybridoma.” Collect. Czech. Chem. Commun. 2003, 68, 2190-2200. doi:10.1135/cccc20032190.
Howell W, G.E. Keller III, J.D. Kirkpatrick, R.L. Jenkins, R.N. Hunsinger and E.W. McLaughlin. “Effects of the plant steroidal hormone, 24-epibrassinolide, on the mitotic index and growth of onion (Allium cepa) root tips.” Genet. Mol. Res. 6 (1): 50-58 (2007).
Ikeda M., Fujiwara S., Mitsuda N. Ohme-Takagi M. (2012) A triantagonistic basic helix-loop-helix system regulates cell elongation in Arabidopsis. Plant Cell Vol. 24 pg. 4483-4497.
Janeczko, A., Biesaga-Kościelniak, J., Oklešt'ková, J., Filek, M., Dziurka, M., Szarek- Łukaszewska, G. and Kościelniak, J. (2010), Role of 24-Epibrassinolide in Wheat Production: Physiological Effects and Uptake. Journal of Agronomy and Crop Science, 196: 311–321. doi: 10.1111/j.1439-037X.2009.00413.x
Malabadi, R.B. and K. Nataraja, 2007. “24-epibrassinolide induces somatic embryogenesis in IPinus wallichiana A. B. Jacks.” J. Plant Sci., 2: 171-178.
DOI: 10.3923/jps.2007.171.178 URL: http://scialert.net/abstract/?doi=jps.2007.171.178
Mousavi E, K. Manochehri Kalantari and S. R. Jafari. “Change of some osmolytes accumulation in water-stressed Colza (Brassica napus L.) As affected by 24-epibrassinolide.” Iranian Journal of Science & Technology, Transaction A, Vol. 33, No. A1.
Samira Ibn Maaouia Houimli, Mounir Denden and Salem Ben El Hadj. “Induction of salt tolerance in pepper (Capsicum annuum) by 24-epibrassinolide.” EurAsia J BioSci 2, 83-90 (2008). Samira Ibn Maaouia Houimli, Mounir Denden and Bouthaina Dridi Mouhandes. “Effects of 24- epibrassinolide on growth, chlorophyll, electrolyte leakage and proline by pepper plants under NaCl-stress.” EurAsia J BioSci 4, 96-104 (2010). DOI:10.5053/ejobios.2010.4.0.12
Zhang L.Y., Bai M.Y., Wu J., Zhu J.Y., Wang H., Zhang Z., Wang W., Sun Y., Zhao J., Sun X., Yang H., Xu Y., Kim S.H., Fujioka S., Lin W.H., Chong K., Lu T., and Wang Z.Y. (2009) Antagonistic HLH/bHLH transcription factors mediate brassinosteroid regulation of cell elongation and plant development in rice and Arabidopsis. Plant Cell Vol. 21 pg. 3767–3780.