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DL-Phosphinothricin Solution (1 mg/mL)
Product ID: G523

Introduction

Description: DL-Phosphinothricin is a herbicide that has been used as a selection agent for the genes bar or pat and is the active ingredient in Basta®.
Synonyms: Glufosinate Ammonium; PPT; 2-amino-4-(hydroxymethylphosphinyl) butanoic acid

Miscibility Water
Physical Form Liquid
Sterility Sterile Filtered
Storage Temp. 2 to 6 C
UPC / SKU G523
CAS NUMBER 77182-82-2
Formula Weight 198.19
Formula C5H15N2O4P
Synonyms Glufosinate Ammonium, PPT, 2-amino-4-(hydroxymethylphosphinyl) butanoic acid
Manufactured from P679
Storage Temp. 2 to 6 °C
Tariff Code 2931.90.9043
Risk Info (R) 60-63-20/21/22-48/20/22
Safety Info (S) 53-45
No information available

G523 DL-Phosphinothricin Solution, 1 mg/mL

Synonyms: PPT; 2-Amino-4-(hydroxymethylphosphinyl)butanoic Acid, Monoammonium Salt, Glufosinate-ammonium
CAS: 77182-82-2
Formula: C5H15N2O4P
Mol. Weight: 198.19
Properties
Form: Aqueous Solution
Appearance: Clear, Colorless Liquid
Application: Molecular Biology
Solubility: Miscible with Water
Storage Temp: 2 – 6 °C
Typical Working Concentration: 0.5 to 400 µg/mL – See Application Notes Below
Other Notes: For Research Use Only

Application Notes
DL-phosphinothricin is also known as glufosinate-ammonium (GLA) and is an active ingredient in Basta®. It functions by inhibiting the glutamine synthase in the chloroplast from synthesizing glutamine to glutamate, which causes ammonia accumulation, thus results in plant death.2, 3, 4

Typical working concentration of GLA varies by applications. It has been reported that treatment of GLA at 0.5 µg/mL stimulates the somatic embryo formation of Vitis interspecific hybrid2 , while GLA concentrations range from 50 µg/mL to 400 µg/mL are used in the transformation of Magnaporthe grisea3

PhytoTechnology Laboratories® also carries DL-Phosphinothricin solution at 1 mg/mL, Product No. G523.

Please Note: It is the sole responsibility of the purchaser to determine the appropriateness of this product for the specific plants that are being cultured and applications that are being used.

References
1. Merck 13, 7425
2. Droge, W., I. Broer, and A. Puhler. 1992. Transgenic plants containing the phosphinothricin-Nacetyltransferase gene metabolize the herbicide L-phosphinothricin (glufosinate) differently from transformed plants. Planta. 187:142-151.
3. Droge-Laser, Wolfgang, Ulrich Siemeling, Alfred Puhler, and Inge Broer. 1994. The metabolites of the herbicide L-phosphinothricin (glufosinate). Plant Physiol. 105:159-166.
4. You, Wenqi and Allen V. Barker. 2002. Herbicidal actions of root-applied glufosinate ammonium on tomato plants. J. Amer. Soc. Hort. Sci. 127(2):200-204.
5. Hebert-Soule D, Kikkert JR, Reisch BI, 1995. Phosphinothricin stimulates somatic embryogenesis in grape (Vitis sp. L.), Plant Cell Reports 14(6):380-384.
6. Leung H, Loomis P, and Pall ML. Transformation of Magnaporthe grisea to phosphinothricin resistance using the bar gene from Streptomyces hygroscopicus. http://www.fgsc.net/fgn42/leung.html (accessed 9 Apr 2014)