Introduction
Description: DL-Phosphinothricin is a herbicide that has been used as a selection agent for the genes bar or pat and is the active ingredient in Basta®.
Synonyms: Glufosinate Ammonium; PPT; 2-amino-4-(hydroxymethylphosphinyl) butanoic acid
| Miscibility |
Water |
| Physical Form |
Liquid |
| Sterility |
Sterile Filtered |
| Storage Temp. |
2 to 6 °C |
| UPC / SKU |
G523 |
| CAS NUMBER |
77182-82-2 |
| Formula Weight |
198.19 |
| Formula |
C5H15N2O4P |
| Synonyms |
Glufosinate Ammonium, PPT, 2-amino-4-(hydroxymethylphosphinyl) butanoic acid |
| Manufactured from |
P679 |
| Storage Temp. |
2 to 6 °C |
| Tariff Code |
2931.90.9043 |
| Risk Info (R) |
60-63-20/21/22-48/20/22 |
| Safety Info (S) |
53-45 |
No information available
G523 DL-Phosphinothricin Solution, 1 mg/mL
| Synonyms: |
PPT; 2-Amino-4-(hydroxymethylphosphinyl)butanoic Acid, Monoammonium Salt, Glufosinate-ammonium |
| CAS: |
77182-82-2 |
| Formula: |
C5H15N2O4P |
| Mol. Weight: |
198.19 |
| Properties |
|---|
| Form: |
Aqueous Solution |
| Appearance: |
Clear, Colorless Liquid |
| Application: |
Molecular Biology |
| Solubility: |
Miscible with Water |
| Storage Temp: |
2 – 6 °C |
| Typical Working Concentration: |
0.5 to 400 µg/mL – See Application Notes Below |
| Other Notes: |
For Research Use Only |
Application Notes
DL-phosphinothricin is also known as glufosinate-ammonium (GLA) and is an active ingredient in Basta®. It functions by inhibiting the glutamine synthase in the chloroplast from synthesizing glutamine to glutamate, which causes ammonia accumulation, thus results in plant death.2, 3, 4
Typical working concentration of GLA varies by applications. It has been reported that treatment of GLA at 0.5 µg/mL stimulates the somatic embryo formation of Vitis interspecific hybrid2 , while GLA concentrations range from 50 µg/mL to 400 µg/mL are used in the transformation of Magnaporthe grisea3
PhytoTechnology Laboratories® also carries DL-Phosphinothricin solution at 1 mg/mL, Product No. G523.
Please Note: It is the sole responsibility of the purchaser to determine the appropriateness of this product for the specific plants that are being cultured and applications that are being used.
References
1. Merck 13, 7425
2. Droge, W., I. Broer, and A. Puhler. 1992. Transgenic plants containing the phosphinothricin-Nacetyltransferase gene metabolize the herbicide L-phosphinothricin (glufosinate) differently from transformed plants. Planta. 187:142-151.
3. Droge-Laser, Wolfgang, Ulrich Siemeling, Alfred Puhler, and Inge Broer. 1994. The metabolites of the herbicide L-phosphinothricin (glufosinate). Plant Physiol. 105:159-166.
4. You, Wenqi and Allen V. Barker. 2002. Herbicidal actions of root-applied glufosinate ammonium on tomato plants. J. Amer. Soc. Hort. Sci. 127(2):200-204.
5. Hebert-Soule D, Kikkert JR, Reisch BI, 1995. Phosphinothricin stimulates somatic embryogenesis in grape (Vitis sp. L.), Plant Cell Reports 14(6):380-384.
6. Leung H, Loomis P, and Pall ML. Transformation of Magnaporthe grisea to phosphinothricin resistance using the bar gene from Streptomyces hygroscopicus. http://www.fgsc.net/fgn42/leung.html (accessed 9 Apr 2014)
