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Hanahan's Broth (SOB Medium)
Product ID: H289

Introduction

Description: Hanahan's Broth (SOB Medium) was developed by Douglas Hanahan (1983) for the cultivation of competentEscherichia coli cells prior to transformation.SOB Medium is a nutrient-rich, bacterial growth medium that is used for microbiological culture, generally of E. coli.

When 20 mM (0.36%) D-glucose is added to this nutritionally rich medium it is referred to as SOC (Super Optimal broth with Catabolite repression) Medium. It is used in the final stage of transformation; this allows the E. coli cells to mend the perforations and replicate.Synonyms: Super Optimal Broth; SOB

Solubility Water
Physical Form Solid
Solutions Available S657 - SOC Medium, 1X (H289 + 20 mM glucose)
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 28.09
UPC / SKU H289
Synonyms Super Optimal Broth; SOB
Storage Temp. 2-6 C
Tariff Code 3821.00.0000
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
M150 Magnesium Sulfate, Anhydrous 2400
P704 Potassium Chloride 186
S624 Sodium Chloride 500
T832 Tryptone 20,000
Y892 Yeast Extract 5000

H289 Hanahan’s Broth (SOB Medium)

Properties
Form: Powder
Appearance: Off-White to Light Tan
Application: Molecular Biology
Solubility: Water
Typical Working Concentration: 28.09 g/L
Storage Temp: 2-6°C
Storage Temp of Stock Solution: Storage of stock solutions is not recommended as precipitates may form.
Other Notes: Approximate pH of 7.0
Formula (mg/L)
Magnesium Sulfate, Anhydrous 2400
Potassium Chloride 186
Sodium Chloride 500
Tryptone 20,000
Yeast Extract 5000

Application Notes
Also known as SOB Medium. It was developed by Hanahan (1983) for the cultivation of competent E. coli cells prior to transformation. When 20 mM (0.36%) D-glucose is added to this nutritionally rich medium it is used in the final stage of transformation; this allows the E. coli cells to mend the performations and replicate

Adding D-glucose to SOB Medium produces SOC Medium (Prod. No. S657).

References
Hanahan, D. (1983) Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 155, 557–508.