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Hosta Rooting Medium
Product ID: H437

Introduction

Description: Stage III medium. Modified Murashige & Skoog (1962) with (mg/L): 0.1 6-BA, 500.0 Casein Hydrolysate, and 0.5 NAA.

Contains the macro- and micronutrients as described by Murashige & Skoog (1962) and the vitamins described by Linsmaier & Skoog (1965). Also contains sucrose and agar.

Solubility Water (Partially)
Physical Form Solid
Storage Temp. 2-6 C
Grams of powder to prepare 1 Liter 43.23
Other Notes Plant Tissue Culture Tested
UPC / SKU H437
Storage Temp. 2-6 C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
A300 Ammonium Nitrate 1650
B210 Boric Acid 6.2
C266 Calcium Chloride, Anhydrous 332.2
C350 Cobalt Chloride Hexahydrate 0.025
C375 Cupric Sulfate, Pentahydrate 0.025
E410 EDTA, Disodium Salt 37.26
F263 Ferrous Sulfate, Heptahydrate 27.8
M150 Magnesium Sulfate, Anhydrous 180.7
M250 Manganese Sulfate 16.9
P840 Potassium Iodide 0.83
P100 Potassium Nitrate 1900
P846 Potassium Phosphate, Monobasic, Anhydrous 300
M651 Sodium Molybdate (VI), Dihydrate 0.25
S515 Sodium Phosphate, Monobasic 170
Z101 Zinc Sulfate, Heptahydrate 8.6
A111 Agar 8000
B800 6-Benzylaminopurine (6-BA) 0.1
C184 Casein, Enzymatic Hydrolysate 500
I703 myo-Inositol 100
N600 a-Naphthaleneacetic Acid 0.5
S391 D-Sucrose 30,000
T390 Thiamine HCl 0.4

H437 Hosta Rooting Medium Stage III Medium

Properties
Form: Powder
Appearance: White to Yellow
Application: Plant Tissue Culture
Solubility: Partially Soluble in Cold Water. Fully Soluble in Hot/Boiling Water.
Typical Working Concentration: 43.23 g/L
Storage Temp: 2-6°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Murashige and Skoog (1962) and the vitamins described by Linsmaier and Skoog (1965). Also contains agar and sucrose. Plant Tissue Culture Tested.
Formula (mg/L)
Ammonium Nitrate 1650
Boric Acid 6.2
Calcium Chloride, Anhydrous 332.2
Cobalt Chloride•6H2O 0.025
Cupric Sulfate•5H2O 0.025
Disodium EDTA •2H2O 37.26
Ferrous Sulfate•7H2O 27.8
Magnesium Sulfate, Anhydrous 180.7
Manganese Sulfate•H2O 16.9
Molybdic Acid (Sodium Salt)• 2H2O 0.25
Potassium Iodide 0.83
Potassium Nitrate 1900
Potassium Phosphate, Monobasic 300
Sodium Phosphate, Monobasic 170
Zinc Sulfate•7H2O 8.6
Agar 8000
6-Benzylaminopurine (BA) 0.1
Casein, Enzymatic Hydrolysate 500
myo-Inositol 100
α-Naphthaleneacetic Acid 0.5
Sucrose 30,000
Thiamine•HCl 0.4

Application Notes
Plant species: Hosta

References
Linsmaier, EM and F Skoog. 1965. Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18: 100-127. Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiologia Plantarum. 15: 473-497.