Hosta Rooting Medium Product ID: H437
Introduction
Description: Stage III medium. Modified Murashige & Skoog (1962) with (mg/L): 0.1 6-BA, 500.0 Casein Hydrolysate, and 0.5 NAA.
Contains the macro- and micronutrients as described by Murashige & Skoog (1962) and the vitamins described by Linsmaier & Skoog (1965). Also contains sucrose and agar.
Solubility |
Water (Partially) |
Physical Form |
Solid |
Storage Temp. |
2-6 °C |
Grams of powder to prepare 1 Liter |
43.23 |
Other Notes |
Plant Tissue Culture Tested |
UPC / SKU |
H437 |
Storage Temp. |
2-6 °C |
Tariff Code |
3822.00.5090 |
Risk Info (R) |
8-36/37/38 |
Safety Info (S) |
17-26-36 |
No information available
PROD. # |
COMPONENT NAME |
mg/L |
A300 |
Ammonium Nitrate |
1650 |
B210 |
Boric Acid |
6.2 |
C266 |
Calcium Chloride, Anhydrous |
332.2 |
C350 |
Cobalt Chloride Hexahydrate |
0.025 |
C375 |
Cupric Sulfate, Pentahydrate |
0.025 |
E410 |
EDTA, Disodium Salt |
37.26 |
F263 |
Ferrous Sulfate, Heptahydrate |
27.8 |
M150 |
Magnesium Sulfate, Anhydrous |
180.7 |
M250 |
Manganese Sulfate |
16.9 |
P840 |
Potassium Iodide |
0.83 |
P100 |
Potassium Nitrate |
1900 |
P846 |
Potassium Phosphate, Monobasic, Anhydrous |
300 |
M651 |
Sodium Molybdate (VI), Dihydrate |
0.25 |
S515 |
Sodium Phosphate, Monobasic |
170 |
Z101 |
Zinc Sulfate, Heptahydrate |
8.6 |
A111 |
Agar |
8000 |
B800 |
6-Benzylaminopurine (6-BA) |
0.1 |
C184 |
Casein, Enzymatic Hydrolysate |
500 |
I703 |
myo-Inositol |
100 |
N600 |
a-Naphthaleneacetic Acid |
0.5 |
S391 |
D-Sucrose |
30,000 |
T390 |
Thiamine HCl |
0.4 |
H437 Hosta Rooting Medium Stage III Medium
Properties |
Form: |
Powder |
Appearance: |
White to Yellow |
Application: |
Plant Tissue Culture |
Solubility: |
Partially Soluble in Cold Water. Fully Soluble in Hot/Boiling Water. |
Typical Working Concentration: |
43.23 g/L |
Storage Temp: |
2-6°C |
Storage Temp of Stock Solution: |
Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
Other Notes: |
Contains the macro- and micronutrients as described by Murashige and Skoog (1962) and the vitamins described by Linsmaier and Skoog (1965). Also contains agar and sucrose. Plant Tissue Culture Tested. |
Formula (mg/L) |
Ammonium Nitrate |
1650 |
Boric Acid |
6.2 |
Calcium Chloride, Anhydrous |
332.2 |
Cobalt Chloride•6H2O |
0.025 |
Cupric Sulfate•5H2O |
0.025 |
Disodium EDTA •2H2O |
37.26 |
Ferrous Sulfate•7H2O |
27.8 |
Magnesium Sulfate, Anhydrous |
180.7 |
Manganese Sulfate•H2O |
16.9 |
Molybdic Acid (Sodium Salt)• 2H2O |
0.25 |
Potassium Iodide |
0.83 |
Potassium Nitrate |
1900 |
Potassium Phosphate, Monobasic |
300 |
Sodium Phosphate, Monobasic |
170 |
Zinc Sulfate•7H2O |
8.6 |
Agar |
8000 |
6-Benzylaminopurine (BA) |
0.1 |
Casein, Enzymatic Hydrolysate |
500 |
myo-Inositol |
100 |
α-Naphthaleneacetic Acid |
0.5 |
Sucrose |
30,000 |
Thiamine•HCl |
0.4 |
Application Notes
Plant species: Hosta
References
Linsmaier, EM and F Skoog. 1965. Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18: 100-127. Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiologia Plantarum. 15: 473-497.
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