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Knudson C Orchid Medium
Product ID: K400

Introduction

Description: Morel modification with the macro- and micronutrients as described by Knudson (1946).

Solubility Water
Physical Form Solid
Storage Temp. 2-6 C
Grams of powder to prepare 1 Liter 22
Other Notes Plant Tissue Culture Tested
UPC / SKU K400
Storage Temp. 2-6 C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
A300 Ammonium Nitrate 500
A305 Ammonium Sulfate 500
C180 Calcium Nitrate 347.2
F263 Ferrous Sulfate, Heptahydrate 25
M150 Magnesium Sulfate, Anhydrous 122.125
M250 Manganese Sulfate 5.682
P704 Potassium Chloride 250
P846 Potassium Phosphate, Monobasic, Anhydrous 250
S391 D-Sucrose 20,000

K400 Knudson C Orchid Medium Morel Modification

Properties
Form: Powder
Appearance: White to Powder
Application: Plant Tissue Culture
Solubility: Soluble in Water
Typical Working Concentration: 22.0 g/L
Storage Temp: 2-6 C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Morel modification with the macro - and micronutrients and sucrose as described by K nudson (1946).
pH = 4.0 - 5.0 . Plant Tissue Culture Test
Formula (mg/L)
Ammonium Nitrate 500
Ammonium Sulfate 500
Calcium Nitrate 347.2
Ferrous Sulfate.7H2O 25
Magnesium Sulfate, Anhydrous 122.13
Manganese Sulfate.H2O 5.68
Potassium Chloride 250
Potassium Phosphate, Monobasic 250
Sucrose 20,000

Application Notes
Plant species: Cymbidium
This medium was originally developed for the germination of Cymbidium orchid s

References
Knudson, L. 1946. A new nutrient solution for germination of orchid seed. A mer. Orchid Soc. Bull. 15: 214 -217.
Morel, GM. 1965. Clonal propagation of orchids by meristem culture. Cymb. Soc. News 20 :3 -11