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Kao & Michayluk Vitamin Solution (100x)
Product ID: K421

Introduction

Description: Kao & Michayluk vitamin solution (100x).
Use at a concentration of 10 mL/L of medium to achieve the proper final concentration.

Miscibility Water
Physical Form Liquid
Solutions Available 100x
Sterility Sterile Filtered
Storage Temp. -20 C
Other Notes Plant Tissue Culture Tested
UPC / SKU K421
Storage Temp. -20 C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
A103 ?-Aminobenzoic Acid 2
A106 L-Ascorbic Acid 200
B140 D-Biotin 1
C186 Calcium Pantothenate 100
C232 Choline Chloride 100
C242 Cyanocobalamin, Vitamin B12 2
F430 Folic Acid 40
I703 myo-Inositol 10,000
N609 Niacinamide 100
P866 Pyridoxine HCl 100
R779 Riboflavin, Vitamin B2 20
T390 Thiamine HCl 100

K421 Kao & Michayluk Vitamin Solution (100x)

Properties
Form: Liquid
Appearance: Clear, Colorless Solution
Application: Plant Tissue Culture
Solubility: Miscible with Water
Typical Working Concentration: 10.0 mL/L of Medium
Storage Temp: --20-0 °C
Other Notes: Contains the vitamins as described by Kao and Michayluk (1975). Sterile Filtered.
Formula (mg/L)
p-Aminobenzoic Acid 2
myo-Inositol 10,000
L-Ascorbic Acid 200
Niacinamide 100
D-Biotin 1
Pyridoxine.HCl 100
D-Calcium Pantothenate 100
Retinol; Vitamin A 1
Choline Chloride 100
Riboflavin 20
Cyancobalamin; Vit B12 2
Thiamine.HCl 100

Application Notes
Plant species: Vicia hajastana (Kao & Michayluk, 1975); Vicia faba (Binding & Nehls, 1978); Daucus carota (Grzebelus et al, 2012).

References
Binding, H. and Nehls, R. (1978) Regeneration of Isolated Protoplasts of Vicia faba L. Z. Pflanzenpbysiol. 88, 327-332.

Grzebelus E, M Szklarczyk & R Baranski (2012) An improved protocol for plant regeneration from leafand hypocotyl-derived protoplasts of carrot. Plant Cell Tiss Organ Cult 109:101-109.

Kao, KN and MR Michayluk (1975) Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126:105-110.