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Linsmaier & Skoog Modified Basal Medium w/ Sucrose & Agar , pH Adjusted & Buffered
Product ID: L452

Introduction

Description: Contains the macro-& micronutrients, & vitamins as described by L & S (1964). With 30 g/L sucrose & 7 g/L agar. pH adjusted and buffered.

This medium is the standard Murashige & Skoog (MS) basal salts supplemented with Linsmaier and Skoog vitamins. The medium is pH adjusted and buffered with MES. Under most circumstances, adjusting the pH is not necessary; simply add water and any desired PGR's, gelling agent, or other supplements. The addition of MES will help to stabilize pH fluctuation during media preparation and culture.

Solubility Partially Soluble in Water
Physical Form Solid
Storage Temp. 2-6 C
Grams of powder to prepare 1 Liter 42.53
Other Notes Plant Tissue Culture Tested
UPC / SKU L452
Storage Temp. 2-6 C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
A300 Ammonium Nitrate 1650
B210 Boric Acid 6.2
C266 Calcium Chloride, Anhydrous 332.2
C350 Cobalt Chloride Hexahydrate 0.025
C375 Cupric Sulfate, Pentahydrate 0.025
E410 EDTA, Disodium Salt 37.26
F263 Ferrous Sulfate, Heptahydrate 27.8
M150 Magnesium Sulfate, Anhydrous 180.7
M250 Manganese Sulfate 16.9
P704 Potassium Chloride 100
P672 Potassium Hydroxide 0.83
P840 Potassium Iodide 1900
P100 Potassium Nitrate 170
M651 Sodium Molybdate (VI), Dihydrate 0.25
Z897 Zinc Nitrate, Hexahydrate 8.6
A111 Agar 7000
I703 myo-Inositol 100
M825 MES, Free Acid 1000
S391 D-Sucrose 30,000
T390 Thiamine HCl 0.4

L452 Linsmaier & Skoog Modified Basal Medium w/ 30 g/L Sucrose & 7 g/L Agar pH Adjusted and Buffered

Synonym: Murashige & Skoog (MS) Medium with Minimal Organics (MSMO)

Properties
Form: Powder
Appearance: White to Yellow
Application: Plant Tissue Culture
Solubility: Soluble in Water
Typical Working Concentration: 42.53 g/L
Storage Temp: 2-6°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by Linsmaier and Skoog (1965)
Formula (mg/L)
Ammonium Nitrate 1650
Boric Acid 6.2
Calcium Chloride, Anhydrous 332.2
Cobalt Chloride.6H2O 0.025
Cupric Sulfate.5H2O 0.025
Na2 EDTA.2H2O 37.26
Ferrous Sulfate.7H2O 27.8
Magnesium Sulfate 180.7
Manganese Sulfate.H2O 16.9
Molybdic Acid (Sodium Salt) .2H2O 0.25
Potassium Hydroxide 100
Potassium Iodide 0.83
Potassium Nitrate 1900
Potassium Phosphate, Monobasic 170
Zinc Sulfate.7H2O 8.6
Agar 7000
MES (Free Acid) 1000
myo-Inositol 100
Sucrose 30,000
Thiamine.HCl 0.4

Application Notes
Plant Tissue Culture Tested
This medium is the standard Murashige & Skoog (MS) basal salts supplemented with Linsmaier and Skoog vitamins. The medium is pH adjusted and buffered with MES. Under most circumstances, adjusting the pH is not necessary; simply add water and any desired PGR’s or other supplements.
The addition of MES will help to stabilize pH fluctuations during media preparation and culture

References
Linsmaier, EM and F Skoog. 1965. Organic growth factor requirements of tobacco tissue culture. Physiol. Plant. 18:100-127.