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Linsmaier & Skoog Modified Basal Medium w/ Sucrose & Agar
Product ID: L467

Introduction

Description: Contains the macro- and micronutrients, and vitamins as described by Linsmaier & Skoog (1964). With 30 g/L sucrose & 7 g/L agar.

Solubility Partially Soluble in Water
Physical Form Solid
Storage Temp. 2-6 C
Grams of powder to prepare 1 Liter 41.43
Other Notes Plant Tissue Culture Tested
UPC / SKU L467
Storage Temp. 2-6 C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
A300 Ammonium Nitrate 1650
B210 Boric Acid 6.2
C266 Calcium Chloride, Anhydrous 332.2
C350 Cobalt Chloride Hexahydrate 0.025
C375 Cupric Sulfate, Pentahydrate 0.025
E410 EDTA, Disodium Salt 37.26
F263 Ferrous Sulfate, Heptahydrate 27.8
M150 Magnesium Sulfate, Anhydrous 180.7
M250 Manganese Sulfate 16.9
P672 Potassium Hydroxide 0.83
P840 Potassium Iodide 1900
P100 Potassium Nitrate 170
M651 Sodium Molybdate (VI), Dihydrate 0.25
Z897 Zinc Nitrate, Hexahydrate 8.6
A111 Agar 7000
I703 myo-Inositol 100
S391 D-Sucrose 30,000
T390 Thiamine HCl 0.4

L467 Linsmaier & Skoog Basal Medium w/ 30 g/L Sucrose and 7 g/L Agar

Synonym: Murashige & Skoog (MS) Medium with Minimal Organics (MSMO

Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 41.43 g/L
Storage Temp: 2-6°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by Linsmaier and Skoog (1965). pH = 4.25 – 5.25
Formula (mg/L)
Ammonium Nitrate 1650
Boric Acid 6.2
Calcium Chloride, Anhydrous 332.2
Cobalt Chloride.6H2O 0.025
Cupric Sulfate.5H2O 0.025
Na2 EDTA.2H2O 37.26
Ferrous Sulfate.7H2O 27.8
Magnesium Sulfate, Anhydrous 180.7
Manganese Sulfate.H2O 16.9
Molybdic Acid (Sodium Salt) .2H2O 0.25
Potassium Iodide 0.83
Potassium Nitrate 1900
Potassium Phosphate, Monobasic 170
Zinc Sulfate.7H2O 8.6
Agar 7000
myo-Inositol 100
Sucrose 30,000
Thiamine.HCl 0.4

Application Notes
Plant Tissue Culture Tested Plant species: Originally tobacco; since then many other species
This medium is the standard Murashige & Skoog (MS) basal salts supplemented with Linsmaier and Skoog vitamins. This medium is a subsequent optimization of the medium developed by Murashige and Skoog.Linsmaier’s research on the optimization of vitamins first described by Murashige as essential. Linsmaier found that certain vitamins were not essential when Thiamine•HCI was optimized from 0.1 to 0.4 mg/L.
Linsmaier also noted that Folic acid, p-Aminobenzoic Acid, L-Glutamine, and Ascorbic Acid all had a positive influence on the growth of Nicotiana callus but was not as essential as Thiamine•HCI and myo-Inositol.

References
Linsmaier, EM and F Skoog. 1965. Organic growth factor requirements of tobacco tissue culture. Physiol. Plant. 18:100-127.