ATZ Labs - a subsidiary of Life Technologies (India) Pvt. Ltd.
Life Technologies India
PhytoTechnology Laboratories

Litvay Basal Salt Mixture
Product ID: L546

Introduction

Description: Contains the macro- and micronutrients as described by Litvay et al. (1981).
Litvay et al. (1981) originally developed the medium to culture both juvenile and mature tissues of Douglas-fir (Pseudotsuga menziesii) and Loblolly pine (Pinus taeda). The original formulation contained 30 g/L sucrose and the following vitamins (mg/L): 100 myo-Inositol, 0.5 Nicotinic Acid, 0.1 Pyridoxine-HCl, and 0.1 Thiamine-HCl.

Solubility Water
Physical Form Solid
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 4.95
Other Notes Plant Tissue Culture Tested
UPC / SKU L546
Storage Temp. 2-6 °C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
A300 Ammonium Nitrate 1650
B210 Boric Acid 31
C266 Calcium Chloride, Anhydrous 16.61
C350 Cobalt Chloride Hexahydrate 0.125
C375 Cupric Sulfate, Pentahydrate 0.5
E676 Ferric Sodium EDTA 36.7
M150 Magnesium Sulfate, Anhydrous 903.38
M250 Manganese Sulfate 21
P672 Potassium Hydroxide 4.15
P840 Potassium Iodide 1900
P100 Potassium Nitrate 340
M651 Sodium Molybdate (VI), Dihydrate 1.25
Z897 Zinc Nitrate, Hexahydrate 43

L546 Litvay Basal Salt Mixture

Synonym: LM Basal Salt Mixture

Properties
Form: Fine Powder
Appearance: Off-white to Yellow
Application: Plant Tissue Culture
Solubility: Soluble in Water
Typical Working Concentration: 4.95g/L
Storage Temp: 2-6°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Litvay et al. (1981).
Formula (mg/L)
Ammonium Nitrate  1650
Boric Acid  31
Calcium Chloride, Anhydrous  16.61
Cobalt Chloride.6H2O  0.125
Cupric Sulfate.5H2O  0.5
Ferric Sodium EDTA  36.7
Magnesium Sulfate, Anhydrous  903.38
Manganese Sulfate.H2O  21
Molybdic Acid (Sodium Salt). 2H2O 1.25
Potassium Iodide  4.15
Potassium Nitrate  1900
Potassium Phosphate, Monobasic  340
Zinc Sulfate.7H2O 43

Application Notes
Plant species: Douglas-fir, Loblolly Pine, Carrot Litvay et al.
(1981) originally developed the medium to culture both juvenile and mature tissues of Douglas-fir (Pseudotsuga menziesii) and Loblolly pine (Pinus taeda). The original formulation contained 30 g/L sucrose and the following vitamins (mg/L): 100 myo-Inositol, 0.5 Nicotinic Acid, 0.1 Pyridoxine•HCl and 0.1 Thiamine•HCl.
This medium was later used to study embryogenesis in wild carrot (Daucus carota L.) suspension cells (Litvay et al, 1985).

References
Litvay, JD, MA Johnson, DC Verma, D Einspahr, K Weyrauch. 1981. Conifer suspension culture medium development using analytical data from developing seeds. Inst.
Paper Chemistry, IPC Tech Paper Ser No 115. Appleton, WI. Litvay, JD, DC Verma, MA Johnson. 1985. Influence of loblolly pine (Pinus taeda L.) culture medium and its components on growth and somatic embryogenesis of the wild carrot (Daucus carota L.). Plant Cell Rep. 4:325.