ATZ Labs - a subsidiary of Life Technologies (India) Pvt. Ltd.
Life Technologies India
PhytoTechnology Laboratories

Murashige & Skoog Modified Basal Medium with Gamborg Vitamins
Product ID: M404

Introduction

Description: Contains the macro & micronutrients described by Murashige & Skoog, & vitamins described by Gamborg, et al (1966).

Solubility Water
Physical Form Solid
Storage Temp. 2-6 ░C
Grams of powder to prepare 1 Liter 4.44
Other Notes Plant Tissue Culture Tested
UPC / SKU M404
Storage Temp. 2-6 ░C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
A300 Ammonium Nitrate 1650
B210 Boric Acid 6.2
C266 Calcium Chloride, Anhydrous 332.2
C350 Cobalt Chloride Hexahydrate 0.025
C375 Cupric Sulfate, Pentahydrate 0.025
E410 EDTA, Disodium Salt 37.26
F263 Ferrous Sulfate, Heptahydrate 27.8
M150 Magnesium Sulfate, Anhydrous 180.7
M250 Manganese Sulfate 16.9
P840 Potassium Iodide 0.83
P100 Potassium Nitrate 1900
P846 Potassium Phosphate, Monobasic, Anhydrous 170
M651 Sodium Molybdate (VI), Dihydrate 0.25
Z101 Zinc Sulfate, Heptahydrate 8.6
I703 myo-Inositol 100
N765 Nicotinic Acid 1.0
P866 Pyridoxine HCl 1.0
T390 Thiamine HCl 10

M404 Murashige & Skoog (MS) Modified Basal Medium w/ Gamborg Vitamins

Properties
Form: Fine to Fluffy Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 4.44g/L
Storage Temp: 2-6░
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Murashige and Skoog (1962) and vitamins as described by Gamborg, et al. (1968). pH = 3.5 – 4.5
Formula (mg/L)
Ammonium Nitrate 1650
áBoric Acid 6.2
Calcium Chloride, Anhydrous 332.2
Cobalt Chloride.6H2O 0.025
Cupric Sulfate.5H2O 0.025
Na2EDTA•2H2O 37.26
Ferrous Sulfate 27.8
Magnesium Sulfate, Anhydrous 180.7
Manganese Sulfate.H2O 16.9
Molybdic Acid (Sodium Salt). 2H2O 0.25
Potassium Iodide 0.83
Potassium Nitrate 1900
Potassium Phosphate, Monobasic 170
Zinc Sulfate•7H2O 8.6
myo-Inositol 100
Nicotinic Acid (Free Acid) 1.0
Pyridoxine•HCl 1.0
Thiamine•HCl 10

Application Notes
Plant Tissue Culture Tested
Plant species: Potato

References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.

Gamborg, OL, RA Miller and K Ojima. 1968. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 151-158.