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Murashige & Skoog Modified Basal Salt Mixture (No Nitrogen, Phosphate, or Potassium)
Product ID: M407

Introduction

Description: Contains the macro- and micronutrients as described by Murashige and Skoog (1962) with the following exceptions: No Ammonium Nitrate, Potassium Nitrate, or Potassium Phosphate monobasic.

Solubility Water
Physical Form Solid
Storage Temp. 2-6 C
Grams of powder to prepare 1 Liter 0.61
Other Notes Plant Tissue Culture Tested
UPC / SKU M407
Storage Temp. 2-6 C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
B210 Boric Acid 6.2
C266 Calcium Chloride, Anhydrous 332.2
C350 Cobalt Chloride Hexahydrate 0.025
C375 Cupric Sulfate, Pentahydrate 0.025
E410 EDTA, Disodium Salt 37.26
F263 Ferrous Sulfate, Heptahydrate 27.8
M150 Magnesium Sulfate, Anhydrous 180.7
M250 Manganese Sulfate 16.9
P840 Potassium Iodide 0.83
M651 Sodium Molybdate (VI), Dihydrate 0.25
Z101 Zinc Sulfate, Heptahydrate 8.6

M407 Murashige & Skoog (MS) Modified Basal Salt Mixture (No Nitrogen, Phosphate, or Potassium)

Properties
Form: Fine to Fluffy Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 0.61 g/L
Storage Temp: 2-6
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Murashige and Skoog (1962) with the following exceptions: No Ammonium Nitrate, Potassium Nitrate, or Potassium Phosphate Monobasic. pH = 3.8-4.8
Formula (mg/L)
Boric Acid 6.2
Calcium Chloride, Anhydrous 332.2
Cobalt Chloride.6H2O 0.025
Cupric Sulfate.5H2O 0.025
Na2EDTA.2H2O 37.26
Ferrous Sulfate.7H2O 27.8
Magnesium Sulfate, Anhydrous 180.7
Manganese Sulfate.H2O 16.9
Molybdic Acid (Sodium Salt). 2H2O 0.25
Potassium Iodide 0.83
Zinc Sulfate.7H2O 8.6

Application Notes
Plant Tissue Culture Tested
Plant species: Numerous, especially herbaceous.
This medium was developed to provide researchers with a base medium when determining the optimum nitrogen, potassium, and phosphorus levels.

References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.