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MG Basal Salt Medium
Product ID: M419

Introduction

Description: Contains the macronutrients as described by Murashige and Skoog (1962) and the micronutrients as described by Gamborg et al. (1968).

Solubility Water
Physical Form Solid
Storage Temp. 2-6 C
Grams of powder to prepare 1 Liter 1.88
Other Notes Plant Tissue Culture Tested
UPC / SKU M419
Storage Temp. 2-6 C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
A305 Ammonium Sulfate 33.5
B210 Boric Acid 2.3
C266 Calcium Chloride, Anhydrous 111.36
C350 Cobalt Chloride Hexahydrate 0.0125
C375 Cupric Sulfate, Pentahydrate 0.0125
E410 EDTA, Disodium Salt 18.64
F263 Ferrous Sulfate, Heptahydrate 13.9
M150 Magnesium Sulfate, Anhydrous 75.7
M250 Manganese Sulfate 6.7
P840 Potassium Iodide 0.4
P100 Potassium Nitrate 1100
P846 Potassium Phosphate, Monobasic, Anhydrous 42.5
M651 Sodium Molybdate (VI), Dihydrate 0.125
S802 Sodium Nitrate 437.8
S515 Sodium Phosphate, Monobasic 32.6
Z101 Zinc Sulfate, Heptahydrate 2.7

M419 MG Basal Salt Mixture Modified Murashige & Skoog/ Gamborg Basal Salt Mixture

Properties
Form: Powder
Appearance White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 1.88 g/L
Storage Temp: 2 - 6 C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macronutrients as described by Murashige and Skoog (1962) and the micronutrients as described by Gamborg et al. (1968)
Formula (mg/L)
Ammonium Sulfate 33.5
Boric Acid 2.3
Calcium Chloride, Anhydrous 111.36
Cobalt Chloride.6H2O 0.0125
Cupric Sulfate.5H2O 0.0125
Na2 EDTA.2H2O 18.64
Ferrous Sulfate.7H2O 13.9
Magnesium Sulfate, Anhydrous 75.7
Manganese Sulfate.H2O 6.7
Molybdic Acid (Sodium Salt).2H2O 0.125
Potassium Iodide 0.4
Potassium Nitrate 1100
Potassium Phosphate, Monobasic 42.5
Sodium Nitrate 437.8
Sodium Phosphate Monobasic 32.6
Zinc Sulfate.7H2O 2.7

Application Notes
Plant Tissue Culture Tested

References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.

Gamborg, OL, RA Miller, K Ojima. 1968. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Research 50: 151-158.