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Murashige & Skoog Modified Basal Salt Mixture
Product ID: M499

Introduction

Description: Contains the macro- and micronutrients as described by Murashige and Skoog (1962) except for the replacement of Ferrous Sulfate and Na2-EDTA with FeNa-EDTA.

Solubility Water
Physical Form Solid
Storage Temp. 2-6 C
Grams of powder to prepare 1 Liter 4.3
Other Notes Plant Tissue Culture Tested
UPC / SKU M499
Storage Temp. 2-6 C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
A300 Ammonium Nitrate 1650
B210 Boric Acid 6.2
C266 Calcium Chloride, Anhydrous 333
C350 Cobalt Chloride Hexahydrate 0.025
C375 Cupric Sulfate, Pentahydrate 0.025
E676 Ferric Sodium EDTA 36.7
M150 Magnesium Sulfate, Anhydrous 181
M250 Manganese Sulfate 16.9
P840 Potassium Iodide 0.83
P100 Potassium Nitrate 1900
P846 Potassium Phosphate, Monobasic, Anhydrous 170
M651 Sodium Molybdate (VI), Dihydrate 0.25
Z101 Zinc Sulfate, Heptahydrate 8.6

M499 Murashige & Skoog (MS) Modified Basal Salt Mixture

Synonym: MS Modified Salts, Contains FeNa-EDTA

Properties
Form: Fine to Fluffy Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 4.30 g/L
Storage Temp: 2-6
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Murashige and Skoog (1962) except for the replacement of Ferrous Sulfate and Disodium EDTA with Ferric Sodium EDTA.
pH = 4.25 – 5.25
Formula (mg/L)
Ammonium Nitrate 1650
Boric Acid 6.2
Calcium Chloride, Anhydrous 333
Cobalt Chloride.6H2O 0.025
Cupric Sulfate.5H2O 0.025
Ferric Sodium EDTA 36.7
Magnesium Sulfate, Anhydrous 181
Manganese Sulfate.H2O 16.9
Molybdic Acid (Sodium Salt). 2H2O 0.25
Potassium Iodide 0.83
Potassium Nitrate 1900
Potassium Phosphate, Monobasic 170
Zinc Sulfate.7H2O 8.6

Application Notes
Plant Tissue Culture Tested

References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.