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Murashige & Skoog Basal Salt Mixture, Finer & Nagasawa Modification
Product ID: M504

Introduction

Description: Finer and Nagasawa Modification.
Contains the macro- and micronutrients as described by Murashige and Skoog (1962) with the following exceptions: Contains 1.6x Potassium Nitrate and 0.5x Ammonium Nitrate; Ferrous Sulfate and Na2-EDTA is replaced with FeNa-EDTA.

Solubility Water
Physical Form Solid
Storage Temp. 2-6 C
Grams of powder to prepare 1 Liter 4.61
Other Notes Plant Tissue Culture Tested
UPC / SKU M504
Storage Temp. 2-6 C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
A300 Ammonium Nitrate 825
B210 Boric Acid 6.2
C266 Calcium Chloride, Anhydrous 332.2
C350 Cobalt Chloride Hexahydrate 0.025
C375 Cupric Sulfate, Pentahydrate 0.025
E676 Ferric Sodium EDTA 36.7
M150 Magnesium Sulfate, Anhydrous 180.54
M250 Manganese Sulfate 16.9
P840 Potassium Iodide 0.83
P100 Potassium Nitrate 3030
P846 Potassium Phosphate, Monobasic, Anhydrous 170
M651 Sodium Molybdate (VI), Dihydrate 0.25
Z101 Zinc Sulfate, Heptahydrate 8.6

M504 Murashige & Skoog (MS) Modified Basal Salt Mixture Finer & Nagasawa Modification

Properties
Form: Fine to Fluffy Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 4.61 g/L
Storage Temp: 2-6
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Murashige and Skoog (1962) with the following exceptions: Contains 1.6x Potassium Nitrate and 0.5x Ammonium Nitrate; Ferrous Sulfate and Disodium EDTA is replaced with Ferric Sodium EDTA.
Formula (mg/L)
Ammonium Nitrate 825
Boric Acid 6.2
Calcium Chloride, Anhydrous 332.2
Cobalt Chloride.6H2O 0.025
Cupric Sulfate.5H2O 0.025
Ferric Sodium EDTA 36.7
Magnesium Sulfate, Anhydrous 180.54
Manganese Sulfate.H2O 16.9
Molybdic Acid (Sodium Salt). 2H2O 0.25
Potassium Iodide 0.83
Potassium Nitrate 3030
Potassium Phosphate, Monobasic 170
Zinc Sulfate.7H2O 8.6

Application Notes
Plant Tissue Culture Tested
Plant species: Soybean

References
Finer JJ, and A Nagasawa. 1988. Development of an embryogenic suspension culture of soybean (Glycine max Merrill.). Plant Cell Tissue Org Cult 15: 125-136.
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.