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Murashige Modified Cattleya Orchid Multiplication Medium
Product ID: M507

Introduction

Description: Contains the macro- and micronutrients as described by Murashige and Skoog (1962) and modified vitamins.
Contains 20 g/L Sucrose. Also contains: 150 mg/L Citric Acid, 0.3 mg/L IAA, 1.75 mg/L IBA, 1.75 mg/L NAA, and Ferric Sodium EDTA in place of Ferrous Sulfate and Disodium EDTA.

Solubility Water
Physical Form Solid
Storage Temp. 2-6 C
Grams of powder to prepare 1 Liter 24.57
Other Notes Plant Tissue Culture Tested
UPC / SKU M507
Storage Temp. 2-6 C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
A300 Ammonium Nitrate 1650
B210 Boric Acid 6.2
C266 Calcium Chloride, Anhydrous 333
C350 Cobalt Chloride Hexahydrate 0.025
C375 Cupric Sulfate, Pentahydrate 0.025
E676 Ferric Sodium EDTA 36.7
M150 Magnesium Sulfate, Anhydrous 181
M250 Manganese Sulfate 16.9
P840 Potassium Iodide 0.83
P100 Potassium Nitrate 1900
P846 Potassium Phosphate, Monobasic, Anhydrous 170
M651 Sodium Molybdate (VI), Dihydrate 0.25
Z101 Zinc Sulfate, Heptahydrate 8.6
C277 Citric Acid, Anyhdrous 150
G503 Glycine 2
I885 Indole-3-acetic Acid 0.3
I538 Indole-3-butyric Acid 1.75
I703 myo-Inositol 100
N600 a-Naphthaleneacetic Acid 1.75
N765 Nicotinic Acid 0.5
P866 Pyridoxine HCl 0.5
S391 D-Sucrose 20,000
T390 Thiamine HCl 10

M507 Murashige Cattleya Orchid Multiplication Medium

Properties
Form: Powder
Appearance: White to Off-white Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 24.57 g/L
Storage Temp: 2-6 C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Murashige and Skoog (1962).
pH = 2.75 - 3.75
Formula (mg/L)
Ammonium Nitrate 1650
Boric Acid 6.2
Calcium Chloride, Anhydrous 333
Cobalt Chloride.6H2O 0.025
Cupric Sulfate.5H2O 0.025
Ferric Sodium EDTA 36.7
Magnesium Sulfate, Anhydrous 181
Manganese Sulfate.H2O 16.9
Molybdic Acid (Sodium Salt) .2H2O 0.25
Potassium Iodide 0.83
Potassium Nitrate 1900
Potassium Phosphate, Monobasic 170
Zinc Sulfate.7H2O 8.6
Citric Acid (Free Acid) Anhydrous 150
Glycine (Free Base) 2
Indole-3-acetic Acid 0.3
Indole-3-butyric Acid 1.75
myo-Inositol 100
a-Naphthaleneacetic Acid 1.75
Nicotinic Acid (Free Acid) 0.5
Pyridoxine.HCl 0.5
Sucrose 20,000
Thiamine.HCl 10

Application Notes
Plant Tissue Culture Tested
Plant species: Cattleya and other epiphytic orchids.

References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.