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Murashige & Skoog Modified BC Potato Medium
Product ID: M516

Introduction

Description: Contains the macro & micronutrients described by Murashige & Skoog, & vitamins described by Gamborg, et al (1966).

Solubility Water
Physical Form Solid
Storage Temp. 2-6 ░C
Grams of powder to prepare 1 Liter 4.41
Other Notes Plant Tissue Culture Tested
UPC / SKU M516
Storage Temp. 2-6 ░C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
A300 Ammonium Nitrate 1650
B210 Boric Acid 6.2
C266 Calcium Chloride, Anhydrous 333
C350 Cobalt Chloride Hexahydrate 0.025
C375 Cupric Sulfate, Pentahydrate 0.025
E676 Ferric Sodium EDTA 36.7
M150 Magnesium Sulfate, Anhydrous 181
M250 Manganese Sulfate 16.9
P840 Potassium Iodide 0.83
P100 Potassium Nitrate 1900
P846 Potassium Phosphate, Monobasic, Anhydrous 170
M651 Sodium Molybdate (VI), Dihydrate 0.25
Z101 Zinc Sulfate, Heptahydrate 8.6
G503 Glycine 2.0
I703 myo-Inositol 100
K750 Kinetin 0.04
N765 Nicotinic Acid 0.5
P866 Pyridoxine HCl 0.5
T390 Thiamine HCl 0.4

M516 Murashige & Skoog Modified BC Potato Basal Medium

Properties
Form: Fine to Fluffy Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 4.44g/L
Storage Temp: 2-6░
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Murashige and Skoog (1962). Contains Ferric Sodium EDTA in place of Ferrous Sulfate and Disodium EDTA. Also contains Kinetin and the vitamins cited below. pH = 4.25 – 5.25
Formula (mg/L)
Ammonium Nitrate 1650
áBoric Acid 6.2
Calcium Chloride, Anhydrous 333
Cobalt Chloride.6H2O 0.025
Cupric Sulfate.5H2O 0.025
Ferric Sodium EDTA 36.7
Magnesium Sulfate, Anhydrous 181
Manganese Sulfate.H2O 16.9
Molybdic Acid (Sodium Salt). 2H2O 0.25
Potassium Iodide 0.83
Potassium Nitrate 1900
Potassium Phosphate, Monobasic 170
Zinc Sulfate•7H2O 8.6
Glycine 2.0
Kinetin 0.04
myo-Inositol 100
Nicotinic Acid (Free Acid) 0.5
Pyridoxine•HCl 0.5
Thiamine•HCl 0.4

Application Notes
Plant Tissue Culture Tested
Plant species: Potato

References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.