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Murashige Modified Basal Multiplication Medium
Product ID: M536

Introduction

Description: Contains the macro- and micronutrients and vitamins as described by Murashige and Skoog (1962). Also contains (mg/L): 170 Sodium Phosphate Monobasic, 80 Adenine Hemisulfate, and 0.4 Thiamine.

Solubility Water
Physical Form Solid
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 4.68
Other Notes Plant Tissue Culture Tested
UPC / SKU M536
Storage Temp. 2-6 °C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
A300 Ammonium Nitrate 1650
B210 Boric Acid 6.2
C266 Calcium Chloride, Anhydrous 332.2
C350 Cobalt Chloride Hexahydrate 0.025
C375 Cupric Sulfate, Pentahydrate 0.025
E410 EDTA, Disodium Salt 37.26
F263 Ferrous Sulfate, Heptahydrate 27.8
M150 Magnesium Sulfate, Anhydrous 180.7
M250 Manganese Sulfate 16.9
P840 Potassium Iodide 0.83
P100 Potassium Nitrate 1900
P846 Potassium Phosphate, Monobasic, Anhydrous 170
M651 Sodium Molybdate (VI), Dihydrate 0.25
S515 Sodium Phosphate, Monobasic 170
Z101 Zinc Sulfate, Heptahydrate 8.6
A545 Adenine Hemisulfate 80
G503 Glycine 2.0
I703 myo-Inositol 100
N765 Nicotinic Acid 0.5
P866 Pyridoxine HCl 0.5
T390 Thiamine HCl 0.4

M536 Murashige Modified Multiplication Basal Medium

Properties
Form: Fine to Fluffy Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 4.68 g/L
Storage Temp: 2-6°
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by Murashige and Skoog (1962). Also contains (mg/L): 170 Sodium Phosphate Monobasic, 80 Adenine Hemisulfate, and 0.4 Thiamine. pH = 3.25-4.25
Formula (mg/L)
Ammonium Nitrate 1650
Boric Acid 6.2
 Calcium Chloride, Anhydrous 332.2
Cobalt Chloride.6H2O 0.025
 Cupric Sulfate.5H2O 0.025
Na2EDTA.2H2O 37.26
 Ferrous Sulfate.7H2O 27.8
Magnesium Sulfate, Anhydrous 180.7
Manganese Sulfate.H2O 16.9
 Molybdic Acid (Sodium Salt).2H2O 0.25
 Potassium Iodide  0.83
 Potassium Nitrate 1900
Potassium Phosphate, Monobasic 170
Sodium Phosphate, Monobasic 170
Zinc Sulfate.7H2O 8.6
Adenine Hemisulfate 80
Glycine (Free Base) 2
 myo-Inositol 100
Nicotinic Acid (Free Acid) 0.5
 Pyridoxine .HCl 0.5
Thiamine .HCl 0.4

Application Notes
Plant Tissue Culture Tested

References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.