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Murashige & Skoog Modified Basal Medium with Sodium Phosphate and Ferric Sodium EDTA
Product ID: P762

Introduction

Description: Contains the macro- and micronutrients and vitamins as described by Murashige and Skoog (1962) with the following exception: Without Potassium Phosphate Monobasic. Also contains (mg/mL): 300 Sodium Phosphate Monobasic, 150 Adenine Hemisulfate, 1000 Casein, and Ferric Sodium EDTA in place of Ferrous Sulfate and Disodium EDTA.

Solubility Water
Physical Form Solid
Storage Temp. 2-6 C
Grams of powder to prepare 1 Liter 5.69
Other Notes Plant Tissue Culture Tested
UPC / SKU M541
Storage Temp. 2-6 C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
A300 Ammonium Nitrate 1650
B210 Boric Acid 6.2
C266 Calcium Chloride, Anhydrous 332.2
C350 Cobalt Chloride Hexahydrate 0.025
C375 Cupric Sulfate, Pentahydrate 0.025
E676 Ferric Sodium EDTA 36.7
M150 Magnesium Sulfate, Anhydrous 180.7
M250 Manganese Sulfate 16.9
P840 Potassium Iodide 0.83
P100 Potassium Nitrate 1900
M651 Sodium Molybdate (VI), Dihydrate 0.25
S515 Sodium Phosphate, Monobasic 300
Z101 Zinc Sulfate, Heptahydrate 8.6
A545 Adenine Hemisulfate 150
C184 Casein, Enzymatic Hydrolysate 1000
G503 Glycine 2.0
I703 myo-Inositol 100
N765 Nicotinic Acid 5.0
P866 Pyridoxine HCl 1.0
T390 Thiamine HCl 0.5

M541 Murashige & Skoog (MS)Modified Basal Medium

Properties
Form: Powder
Appearance: White to Yellow
Application: Plant Tissue Culture
Solubility: Soluble in Water
Typical Working Concentration: 5.69 g/L
Storage Temp: 2-6C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by Murashige and Skoog (1962) with the following exceptions: Potassium Phosphate Monobasic is omitted, Sodium Phosphate Monobasic is added, and Ferrous Sulfate and Disodium EDTA are replaced with Ferric Sodium EDTA. Approx. pH = 4.75 ± 0.5
Formula (mg/L)
Ammonium Nitrate 1650
Boric Acid 6.2
Calcium Chloride, Anhydrous 332.2
Cobalt Chloride•6H2O 0.025
Cupric Sulfate•5H2O 0.025
Ferric Sodium EDTA 36.7
Magnesium Sulfate, Anhydrous 180.7
Manganese Sulfate•H2O 16.9
Molybdic Acid (Sodium Salt)•2H2O 0.25
Potassium Iodide 0.83
Potassium Nitrate 1900
Sodium Phosphate Monobasic 300
Zinc Sulfate•7H2O 8.6
Adenine Hemisulfate 150
Casein, Enzymatic Hydrolysate 1000
Glycine (Free Base) 2
myo-Inositol 100
Nicotinic Acid (Free Acid) 5
Pyridoxine•HCl 1
Thiamine•HCl 0.5

Application Notes
Plant Tissue Culture Tested

References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497