ATZ Labs - a subsidiary of Life Technologies (India) Pvt. Ltd.
Life Technologies India
PhytoTechnology Laboratories

Malmgren Orchid Medium
Product ID: M551

Introduction

Description: Contains the macro- and micronutrients, agar, and organic constituents as described by Malmgren 1996. Without sucrose.

Solubility Water
Physical Form Solid
Storage Temp. 2-6 C
Grams of powder to prepare 1 Liter 28.84
Other Notes Plant Tissue Culture Tested
UPC / SKU M551
Storage Temp. 2-6 C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
C274 Calcium Phosphate, Tribasic 75
E410 EDTA, Disodium Salt 37.26
F263 Ferrous Sulfate, Heptahydrate 27.8
M150 Magnesium Sulfate, Anhydrous 97.69
M250 Manganese Sulfate 1.54
P846 Potassium Phosphate, Monobasic, Anhydrous 75
C325 Activated Charcoal 1000
A111 Agar 7000
B140 D-Biotin 0.05
C184 Casein, Enzymatic Hydrolysate 400
F430 Folic Acid 0.5
G503 Glycine 2
I703 myo-Inositol 100
N765 Nicotinic Acid 5
P862 Pineapple Powder 20,000
P866 Pyridoxine HCl 5
T390 Thiamine HCl 10

M551 Malmgren Modified Terrestrial Orchid Medium Without Sucrose

Properties
Form: Powder
Appearance: Gray powder
Application: Orchid Culture
Solubility: Water
Typical Working Concentration: 28.84 g/L
Storage Temp: 2-6 C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients, agar, and organic constituents as described by Malmgren (1996) pH = 3.75 - 4.75
Formula (mg/L)
Calcium Phosphate, Tribasic 75
Na2 EDTA.2H2O 37.26
Ferrous Sulfate.7H2O 27.8
Magnesium Sulfate, Anhydrous 97.69
Manganese Sulfate.H2O 1.54
Potassium Phosphate, Monobasic 75
Activated Charcoal 1000
Agar 7000
D-Biotin 0.05
Casein, Enzymatic Hydrolysate 400
Folic Acid 0.5
Glycine (Free Base) 2
myo-Inositol 100
Nicotinic Acid (Free Acid) 5
Pineapple Powder 20,000
Pyridoxine.HCl 5
Thiamine.HCl 10

Application Notes
Plant Tissue Culture Tested
Plant species: Terrestrial or chids including Paphiopedilum, Cypripedium, and Phragmipedium This medium is a good seed sowing medium for many terrestrial orchid species. Originally developed for Cypripedium, this medium is al so used for germination of Phragmipedium and Paphiopedilum. Germination may be enhanced by in cluding 50 ml of Coconut Water (C195) per liter of medium.

References
Malmgren, S. 1996. In: C. Allen, Editor, North Americ an Native Terrestrial Orchids. Propagation and Production C onf. Proc., pp 63-71.