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Murashige & Skoog Medium with Vitamins and 30 g/L Sucrose (1X)
Product ID: M5530

Introduction

Description: Sterile filtered liquid solution containing the Macro- and Micronutrients and Vitamins as described by Murashige & Skoog (1962), then modified to contain 15 g/L sucrose and 15 g/L glucose.

Solubility Water
Physical Form Liquid
Sterility Sterile Filtered
Storage Temp. 2-6 °C
Other Notes Plant Tissue Culture Tested
UPC / SKU M5530
Storage Temp. 2-6 C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
A300 Ammonium Nitrate 1650
B210 Boric Acid 6.2
C266 Calcium Chloride, Anhydrous 332.2
C350 Cobalt Chloride Hexahydrate 0.025
C375 Cupric Sulfate, Pentahydrate
E410 EDTA, Disodium Salt 37.26
F263 Ferrous Sulfate, Heptahydrate 27.8
M150 Magnesium Sulfate, Anhydrous 180.7
M250 Manganese Sulfate 16.9
P840 Potassium Iodide 0.83
P100 Potassium Nitrate 1900
P846 Potassium Phosphate, Monobasic, Anhydrous 170
M651 Sodium Molybdate (VI), Dihydrate 0.25
Z101 Zinc Sulfate, Heptahydrate 8.6
G503 Glycine 2
I703 myo-Inositol 100
N765 Nicotinic Acid 0.5
P866 Pyridoxine HCl 0.5
S391 D-Sucrose 30,000
T390 Thiamine HCl 0.1

M5530 Murashige & Skoog (MS) Basal Medium Solution w/ Vitamins& Sucrose (1X)

Synonym: MS Medium

Properties
Form: Liquid
Appearance: Clear, Colorless to Light Yellow
Application: Plant Tissue Culture
Solubility: Miscible with Water
Typical Working Concentration: 1x
Storage Temp: 2-6C
Other Notes: Contains the macro- and micronutrients and vitamins as described by Murashige and Skoog (1962).
pH = 3.5-4.5
Formula (mg/L)
Ammonium Nitrate 1650
Boric Acid 6.2
Calcium Chloride, Anhydrous 332.2
Cobalt Chloride.6H2O 0.025
Cupric Sulfate.5H2O 0.025
Na2EDTA.2H2O 37.26
Ferrous Sulfate.7H2O 27.8
Magnesium Sulfate, Anhydrous 180.7
Manganese Sulfate.H2O 16.9
Molybdic Acid (Sodium Salt). 2H2O 0.25
Potassium Iodide 0.83
Potassium Nitrate 1900
Potassium Phosphate, Monobasic 170
Zinc Sulfate.7H2O 8.6
Glycine (Free Base) 2
myo-Inositol 100
Nicotinic Acid (Free Acid) 0.5
Pyridoxine.HCl 0.5
Thiamine.HCl 0.1
Sucrose 30,000

Application Notes
Plant Tissue Culture Tested
Plant species: This medium is used for a numerous species.

References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.