mRNA-In« Neuro

Messenger RNA Delivery into Neurons 
mRNA-In« Neuro Transfection Reagent is a next generation low-toxicity mRNA delivery system for achieving maximum transfection efficiency in hard-to-transfect neurons. It is well suited for various neurobiology applications, including neurotoxicity and neurite outgrowth development.




Feature Benefits
  • Maximum efficiency with low amounts of mRNA - Maximally effective with very low amounts of mRNA, significantly minimizing potential adverse off-target cytotoxic effects and supporting maximum cell health and viability.
  • High expression, low toxicity - Achieves high mRNA efficiency and protein expression in hard to transfect neurons while maintaining optimal cell health for uncompromised post-transfection assay results.
  • Versatile performance - Highly efficient and reproducible transfection performance across a wide range of primary cells.
  • Rapid expression and easy to use - Use without the risk of integration with the cellular host genome and observe more rapid expression as early as 4 hours post-transfection.
  • Well suited for various applications - Shown to be effective across a range of applications, including neurotoxicity and neurite development
Low Amounts of mRNA Achieve Maximum RNA Delivery in Neurons

Superior mRNA Transfection efficiency in Primary Neurons – Primary rat cortical neurons cultured in 24-well plates were transfected with mRNA-In« Neuro Transfection Reagent and incubated overnight in complete culture media. Maximum transfection efficiency was maintained with reducing amounts of mRNA, from 500ng (high) to 125ng (low) amounts of mRNA. The above images were taken 48-hours post-transfection. In contrast to DNA transfection, neuronstransfected with mRNA-In« Neuro showed uniform GFP expression from high (500ng) to very low (125ng) amounts of mRNA.

mRNA-In« Neuro Shows Superior Performance over Competitor

mRNA-In« Neuro Outperforms Competitor Reagents - mRNA-In Neuro Transfection Reagent was compared to MessengerMax™ for transfecting neurons. Primary cortical neurons in culture for 6 days were transfected with 500ng and 250ng amounts of modified mRNA and 2Ál mRNA-In« Neuro or MessengerMax™ in 24-well plates. Cells were incubated overnight in complete culture media and observed 24hr and48hrs (not shown) post-transfection. Above images show significantly higher transfection efficiency and GFP expression in healthy neurons post transfection

MessengerMax™ is a product of Thermo Fisher Scientific Inc.

References
    1. Tsai HD, Wu JS, Kao MH, Chen JJ, Sun GY, Ong WY, Lin TN. (2016) Clinacanthus nutans Protects Cortical Neurons Against Hypoxia-Induced Toxicity by Downregulating HDAC1/6 Neuromolecular Med May 10.
    2. Gervasi NM, Scott SS, Aschrafi A, Gale J, Vohra SN, MacGibeny MA, Kar AN, Gioio AE, Kaplan BB. (2016)  The Local Expression and Trafficking Of Tyrosine Hydroxylase mRNA In The Axons Of Sympathetic Neurons RNA 22:1–13.