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Nitsch & Nitsch Vitamin Powder (1000x)
Product ID: N608

Introduction

Description: Contains the vitamins as described by Nitsch and Nitsch (1969).
Instructions for dissolving the powder: Add 5 mL 1N KOH or NaOH per 100 mL package or approx 13 mL per 250 mL package. Stir until suspension dissolves; add additional base if necessary. Bring to volume with water.
This product can be filter sterilized and stored in the refrigerator. Alternatively, it can be stored in aliquots in the freezer for long-term. Freezer storage of non-sterile solutions is preferred as vitamins are subject to breakdown by bacteria and fungi. myo-Inositol may precipitate out of solution when cold; warming with occasional agitation will redissolved the precipitate.

Solubility Water
Physical Form Solid
Solutions Available N603
Concentration 1000x
Storage Temp. 2-6 C
Grams of powder to prepare 1 Liter 108.6
Other Notes Plant Tissue Culture Tested
UPC / SKU N608
Storage Temp. 2-6 C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
B140 D-Biotin 50
F430 Folic Acid 500
G503 Glycine 2000
I703 myo-Inositol ######
N765 Nicotinic Acid 5000
P866 Pyridoxine HCl 500
T390 Thiamine HCl 500

N608 Nitsch & Nitsch Vitamin Powder (1000x)

Properties
Form: Powder
Appearance: White to Off-white powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 10.86 g/ 100 mL produces a 1000X concentrate; 1 mL/ L of medium when dissolved in 1N KOH and water to the volume indicated on the package. See instructions below.
Storage Temp: 2 - 6 C
Storage Temp of Stock Solution: 2 - 6 C
Other Notes: Contains the vitamins as described by Nitsch and Nitsch (1969).
Instructions for dissolving the powder: Add 5 mL 1N KOH or NaOH per 100 mL package or approx 13 mL per 250 mL package. Stir till suspension dissolves; add additional base if necessary. Bring to volume with water.
This product can be filter sterilized and stored in the refrigerator.
Alternatively, it can be stored in aliquots in the freezer for long-term.
Freezer storage of non-sterile solutions is preferred as vitamins are subject to breakdown by bacteria and fungi.
Myo-Inositol may precipitate out of solution when cold; warming with occasional agitation will redissolved the precipitate.
Formula (mg/L)
D-Biotin 50
Folic Acid 500
Glycine (Free Base) 2000
myo-Inositol 100,000
Nicotinic Acid (Free Acid) 5000
Pyridoxine.HCl 500
Thiamine.HCl 500

Application Notes
Plant Tissue Culture Tested

References
Nitsch, JP and C Nitsch. 1969. Haploid plants from pollen grains. Science 163: 85-87.