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Life Technologies India
PhytoTechnology Laboratories

Orchid Multiplication Medium without Charcoal & Agar
Product ID: P793

Introduction

Description: This medium is a modification of our Orchid Multiplication Medium (P723) by the deletion of charcoal and agar. It was originally developed for the multiplication of plantlets from Phaleonopsis flower stem nodal segments. This medium when used in combination with Orchid Maintenance/ Replate Medium (P668 or P748) provides a complete plant propagation cycle.

This medium has been used for protocorm production of Cattleya and Cymbidium species. The pH of this medium should be adjusted after adding a gelling agent and prior to sterilization.

Solubility Water
Physical Form Solid
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 25.3
UPC / SKU P793
Storage Temp. 2-6 °C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
A300 Ammonium Nitrate 825
B210 Boric Acid 3.1
C266 Calcium Chloride, Anhydrous 166
C350 Cobalt Chloride Hexahydrate 0.0125
C375 Cupric Sulfate, Pentahydrate 0.0125
E410 EDTA, Disodium Salt 37.3
F263 Ferrous Sulfate, Heptahydrate 27.85
M150 Magnesium Sulfate, Anhydrous 90.35
M250 Manganese Sulfate 8.45
P840 Potassium Iodide 0.415
P100 Potassium Nitrate 950
P846 Potassium Phosphate, Monobasic, Anhydrous 85
M651 Sodium Molybdate (VI), Dihydrate 0.125
Z101 Zinc Sulfate, Heptahydrate 5.3
B800 6-Benzylaminopurine (6-BA) 2.0
I703 myo-Inositol 100
M825 MES, Free Acid 1000
N600 a-Naphthaleneacetic Acid 0.5
N765 Nicotinic Acid 0.5
P775 Peptone from Meat 2000
P866 Pyridoxine HCl 0.5
S391 D-Sucrose 20,000
T390 Thiamine HCl 1.0

P793 Orchid Multiplication Medium Without Charcoal and Agar

Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Orchid Cultur
Solubility: Water
Typical Working Concentration: 25.30 g/L
Storage Temp: 2-6° C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: pH = 4.75 - 5.75
Formula (mg/L)
Ammonium Nitrate  825
Boric Acid  3.1
Calcium Chloride, Anhydrous  166
Cobalt Chloride.6H2O  0.0125
Cupric Sulfate.5H2O  0.0125
Na2EDTA.2H2O  37.3
Ferrous Sulfate.7H2O  27.85
Magnesium Sulfate,Anhydrous  90.35
Manganese Sulfate.H2O  8.45
Molybdic Acid, Sodium Salt.2H2O  0.125
Potassium Iodide  0.415
Potassium Nitrate  950
Potassium Phosphate, Monobasic 85
Zinc Sulfate.7H2O 5.3
6-Benzylaminopurine (BA) 2
MES (Free Acid) 1000
myo-Inositol 100
a-Naphthaleneacetic Acid 0.5
Nicotinic Acid (Free Acid) 0.5
Peptone from Meat 2000
Pyridoxine.HCl 0.5
Sucrose  20,000
Thiamine.HCl 1

Application Notes
Plant Tissue Culture Tested
Plant species: Many epiphytic orchid species
This medium is a modification of our Orchid Mu ltiplication Medium (P72 3) by the deletion of charcoal and agar. It was or iginally developed for the mult iplication of plantlets from Phalaenopsis flower stem nodal s egments. This medium when us ed in combination with Orchid Maintenance/ Replate Medium (P668 or P748) prov ides a complete plant propagation cycle. This medium has been used for protocorm pr oduction of Cattleya and Cymbidium species. The pH of this medium should be adjusted after adding a gelling agent and prior to sterilization. (Revised 12/2012)