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Rugini Olive Medium
Product ID: R7100

Introduction

Description: Rugini Olive Medium is useful for the micropropagation of some types of olive cultivars (Rugini 1984).
It contains the macronutrients, micronutrients, and vitamins as described by E. Rugini in 1984.

Solubility Water
Physical Form Solid
Storage Temp. 2-6 C
Grams of powder to prepare 1 Liter 4.05
Other Notes Plant Tissue Culture Tested
UPC / SKU R7100
Storage Temp. 2-6 C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
A300 Ammonium Nitrate 412
B210 Boric Acid 12.4
C266 Calcium Chloride, Anhydrous 332.16
C180 Calcium Nitrate 416.9
C350 Cobalt Chloride Hexahydrate 0.025
C375 Cupric Sulfate, Pentahydrate 0.25
E410 EDTA, Disodium Salt 37.5
F263 Ferrous Sulfate, Heptahydrate 27.8
M150 Magnesium Sulfate, Anhydrous 732.5
M250 Manganese Sulfate 16.9
P704 Potassium Chloride 500
P840 Potassium Iodide 0.83
P100 Potassium Nitrate 1100
P846 Potassium Phosphate, Monobasic, Anhydrous 340
M651 Sodium Molybdate (VI), Dihydrate 0.25
Z101 Zinc Sulfate, Heptahydrate 14.3
B140 D-Biotin 0.05
F430 Folic Acid 0.5
G503 Glycine 2
I703 myo-Inositol 100
N765 Nicotinic Acid 5
P866 Pyridoxine HCl 0.5
T390 Thiamine HCl 0.5

R7100 Rugini Olive Medium

Properties
Form: Powder
Appearance: White to Light Yellow
Application: Plant Tissue Culture
Solubility: Soluble in Water
Typical Working Concentration: 4.05g/L
Storage Temp: 2-6°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by E. Rugini (1984). Plant Tissue Culture Tested Unadjusted pH (approx): 3.5 - 4.5
Formula (mg/L)
Ammonium Nitrate 412
Boric Acid 12.4
Calcium Chloride Anhydrous 332.16
Calcium Nitrate, Anhydrous 416.9
Cobalt Chloride 6H2O 0.025
Cupric Sulfate 5H2O 0.25
Na2 EDTA 2H2O 37.5
Ferrous Sulfate 7H2O 27.8
Magnesium Sulfate Anhydrous 732.5
Manganese Sulfate H2O 16.9
Molybdic Acid, Na Salt 2H2O 0.25
Potassium Chloride 500
Potassium Iodide 0.83
Potassium Nitrate 1100
Potassium Phosphate, Monobasic 340
Zinc Sulfate 7H2O 14.3
D-Biotin 0.05
Folic Acid 0.5
Glycine 2
myo-Inositol 100
Nicotinic Acid 5
Pyrodoxine HCl 0.50
Thiamine HCl 0.50

Application Notes
Useful for the micropropagation of some types of olive cultivars (Rugini 1984).

References
Rugini E (1984) In vitro propagation of some olive (Olea europaea L.) cultivars with different root ability, and medium development using analytical data from developing shoots and embryos. Scientia Horticulturae 24: 123-134.