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Staba Modified Vitamin Solution (100x)
Product ID: S743

Introduction

Description: Staba Modified Vitamin Solution (100x) contains a modification of the Staba (1969) vitamins as described by Skirvin and Chu (1979). This modification differs from the original Staba formulation in that myo-Inositol was reduced from 2000 mg/L to 100 mg/L. The original use of these vitamins appears to be for the culture of Digitalis. These vitamins have been used for the culture of Rubus (Blackberry), Prunus (Peach), Rosa (Rose), Cornus (Dogwood), Rhipsalidopsis (Easter Cactus), and Schlumbergera (Christmas Cactus). myo-Inositol may precipitate out of the solution when cold; warming with occasional agitation will redissolve it. Use at a concentration of 10 mL/L of medium to achieve the proper final concentration.

Miscibility Water
Physical Form Liquid
Concentration 100x
Sterility Sterile Filtered
Storage Temp. -20-0 °C
Other Notes Plant Tissue Culture Tested
UPC / SKU S743
Storage Temp. 2-6 C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
A103 ?-Aminobenzoic Acid 50
B140 D-Biotin 100
C186 Calcium Pantothenate 100
C232 Choline Chloride 100
C242 Cyanocobalamin, Vitamin B12 0.15
F430 Folic Acid 50
I703 myo-Inositol 10,000
N609 Niacinamide 200
P866 Pyridoxine HCl 200
R779 Riboflavin, Vitamin B2 50
T390 Thiamine HCl 100

S743 Staba Modified Vitamin Solution (100x)

Properties
Form: Liquid
Appearance: Clear Solution
Application: Plant Tissue Culture
Solubility: Miscible with Water
Typical Working Concentration: 1 mL/ L of medium
Storage Temp: -20-0° C
Storage Temp of Stock Solution: N/A
Other Notes: Contains a modification of the Staba (1969) vitamins as described by Skirvin and Chu (1979). This modification differs from the original Staba formulation in that myo-inositol was reduced from 2000 mg/L to 100 mg/L.
Myo-Inositol may precipitate out of the solution when cold; warming with occasional agitation will redissolved it.
Formula (mg/L)
?-Aminobenzoic Acid 50
D-Biotin 100
Calcium Pantothenate 100
Choline Chloride 100
Cyanocobalamin 0.15
Folic Acid 50
myo-Inositol 10,000
Niacinamide 200
Pyridoxine HCl 200
Riboflavin 50
Thiamine HCl 100

Application Notes
Plant Tissue Culture Tested
The original use of these vitamins appears to be for the culture of Digitalis. These vitamins have been used for the culture of Rubus (Blackberry), Prunus (Peach), Rosa (Rose), Cornus (Dogwood), Rhipsalidopsis (Easter Cactus), and Schlumbergera (Christmas Cactus).

References
Staba, JE. 1969. Plant tissue culture as a technique for the phytochemist. Recent Adv. in Phytochem., 2:80.
Skirvin, RM and MC Chu. 1979. In vitro culture of 'Forever Yours' rose. HortSci., 14: 608-610. (Revised 5/2007)