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PhytoTechnology Laboratories

Sueoka's High Salt Medium (HSM)
Product ID: T8224

Introduction

Description: Tris-acetate-phosphate medium (TAP) is a standard maintenance medium often used for Chlamydomonas reinhardtii, the most well-characterized eukaryotic freshwater algae. Ammonium (NH4+) serves as the primary nitrogen source and Tris buffers the pH. Since TAP contains a relatively low concentration of phosphate, it can be used for P labeling as well as experiments/isolations that require clarity of solid-substrate cultures (e.g. agar) (Harris 1989).

See product info sheet for more information about media preparation and other usage.

Solubility Water
Physical Form Solid
Solutions Available T8050 (1X)
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 3.17
Other Notes Requires 1 mL/L Glacial Acetic Acid (Product # A256)
UPC / SKU T8224
Synonyms Tris-Minimal Medium (if acetate is not added)
Storage Temp. 2-6 C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
A109 Ammonium Chloride 400
A112 Ammonium Molybdate, Tetrahydrate 1.10
B210 Boric Acid 11.40
C266 Calcium Chloride, Anhydrous 37.74
C350 Cobalt Chloride Hexahydrate 1.610
C375 Cupric Sulfate, Pentahydrate 1.570
E410 EDTA, Disodium Salt 50.00
F263 Ferrous Sulfate, Heptahydrate 4.990
M150 Magnesium Sulfate, Anhydrous 48.83
M455 Manganese Chloride, Tetrahydrate 5.060
P705 Potassium Phosphate, Dibasic 108.0
P846 Potassium Phosphate, Monobasic,Anhydrous 54.0
Z101 Zinc Sulfate, Heptahydrate 22.00
T838 Tris Base 2420