Introduction
Description: Acetosyringone is a naturally occurring compound secreted from wounded dicot plant tissues and is a chemotractant for Agrobacterium sp. It is generally used at 5 uM to 200 uM to enhance transformation in monocots.Acetosyringone should NOT be autoclaved. This is a sterile solution of Acetosyringone in DMSO.
Synonym: 3',5'-Dimethyoxy-4'-hydroxyacetophenone
| Autoclavability |
Do not autoclave |
| Miscibility |
Water |
| Physical Form |
Liquid |
| Powder Form |
A1104 |
| Sterility |
DMSO is considered a sterilant |
| Storage Temp. |
-20 to 0 °C |
| Other Notes |
Plant Tissue Culture Tested |
| UPC/SKU |
A1104 |
| CAS NUMBER |
2478-38-8 |
| Formula Weight |
196.20 |
| Formula |
C10H12O4 |
| Synonyms |
3',5'-Dimethyoxy-4'-hydroxyacetophenone |
| Manufactured from |
A104 |
| Storage Temp. |
-20 to 0 °C |
| Tariff Code |
2914.50.3000 |
| Risk Info (R) |
22-36/37/38 |
| Safety Info (S) |
24/25-36 |
No information available
A1104 Acetosyringone 100 mM Solution
| Synonyms: |
4-Hydroxy-3’,5’-dimethoxyacetophenone |
| CAS: |
2478-38-8 |
| Formula: |
C10H12O4 |
| Mol. Weight: |
196.20 |
| Properties |
|---|
| Form: |
Liquid |
| Appearance: |
Off-white to Tan |
| Application: |
Molecular Biology |
| Solubility: |
Miscible in Water |
| Typical Working Concentration: |
50 µM to 200 µM |
| Storage Temp: |
-20 to 0 oC |
| Storage Temp of Stock Solution: |
N/A |
| Other Notes: |
Plant Tissue Culture Tested |
Application Notes
This material is sensitive to temperature and should be added after autoclaved media has cooled slightly. Acetosyringone is a naturally occurring compound secreted from wounded plant tissues. It is used to increase the rate of transformation when used at 50 µM to 200 µM. (Sheikholeslam & Weeks, 1987).
References
Sheikholeslam, SN & DP Weeks. 1987. Acetosyringone promotes high efficiency transformation of Arabidopsis thaliana explants by Agrobacterium tumefaciens. Plant Molecular Biology 8:291- 298.
