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Aniline Blue
Product ID: A1272


Description: A biological (histological) stain used to stain fungi, often used with lactic acid during seed testing. Also used to stain callose structures in plant tissues.
Synonyms: Aniline Bue WS; Water Blue; C.I. 42755; Acid blue 22; Soluble Blue 3M; Marine Blue V; China blue; Soluble blue.

Solubility Water
Storage Temp. Room Temperature
UPC / SKU A1272
CAS NUMBER 28631-66-5
Formula Weight 737.72
Formula C32H25N3O9S3Na2
Synonyms Aniline Bue WS; Water Blue; C.I. 42755; Acid blue 22; Soluble Blue 3M; Marine Blue V; China blue; Soluble blue
Storage Temp. Room Temperature
Tariff Code 3219.90.9000
Risk Info (R) 36/37/38
Safety Info (S) 36/37/39
No information available

A1272 Aniline Blue

Synonyms: Water Blue, Acid blue 22, Soluble Blue 3M, Marine Blue V, or C.I. 42755
CAS: 28631-66-5
Formula: C32H25N3O9S3Na2
Mol. Weight: 737.72
Form: Powder
Appearance: Dark Red to Purple
Solubility: Soluble in Water
Application: Biological Stain, Seed Testing
Storage Temp: Room Temperature
Typical Working Concentration:: Varies with application, should be determined by end user

Application Notes
Aniline blue is often used in plant tissue and seed testing to detect the presence of mycelium (Clark et al., 1983; Hsiang and A. Mueller, 1992). One common use of aniline blue in seed testing is for the detection of the endophyte Neotyphodium coenophialum in tall fescue (USDA, 2008). Other plant tissues, such as Glycine Max (soybean) can be stained with aniline blue to detect Fusarium oxysporum (Agarwal & Sinclair 1996).

Agarwal VK & JB Sinclair (1996) Principles of Seed Pathology, Second Edition. CRC Press, Boca Raton, Fla. USA. Bacon, C.W. & J.F. White (1994) Stains, media and procedures for analyzing endo-phytes, p. 47-56. In C.W. Bacon & J.F. White (eds.), Biotechnology of endophytic fungi of grasses. CRC Press, Boca Raton, Fla. USA. Clark, E.M., White, J.F., Patterson, R.M. (1983) Improved histological techniques for the detection of Acremonium coenophialum in tall fescue and methods of in vitro culture of the fungus. J. Micro. Methods 1:149-155. Hsiang T and A. Mueller (1992) Detection of Endophytes in Tall Fescue. Guelph Turfgrass Institute Annual Research Report Pp. 118-120. USDA (2008) Items of Interest in Seed. Vinton M., E. Kathol, K. Vogel and A. Hopkins (2001) Endophytic Fungi in Canada Wild Rye in Natural Grasslands. Journal of Range Management, 54(4), pp. 390-395.