Introduction
Description: A biological stain useful for staining pectins in plant cell walls.Synonyms: Alcian Blue 8GX, Ingrain Blue 1, C.I. 74240
| Solubility |
Water |
| Physical Form |
Solid |
| Storage Temp. |
Room Temperature |
| UPC / SKU |
A1286 |
| CAS NUMBER |
33864-99-2 |
| Formula Weight |
1298.86 |
| Formula |
C56H68Cl4CuN16S4 |
| Synonyms |
Alcian Blue 8GX, Ingrain Blue 1, C.I. 74240 |
| Storage Temp. |
Room Temperature |
| Tariff Code |
3204.90.0000 |
| Risk Info (R) |
36/37/38 |
| Safety Info (S) |
36/37/39 |
No information available
A1286 Alcian Blue
| Synonyms: |
Alcian Blue 8GX, Ingrain Blue 1, and C.I. 74240 |
| CAS: |
33864-99-2 |
| Formula: |
C56H68Cl4CuN16S4 |
| Mol. Weight: |
1298.86 |
| Properties |
|---|
| Form: |
Powder |
| Appearance: |
Blue or Greenish-Black to Purple |
| Application: |
Biological Stain, Phycology |
| Solubility: |
Soluble in Water |
| Typical Working Concentration: |
Varies with Application, should be determined by end user |
| Storage Temp: |
Room Temperature |
Application Notes
A widely used cationic dye used to stain acid mucopolysaccharides and glycosaminoglycans. The stained tissues are blue to bluish-green in color. Particularly useful in staining pectins present in plant cell walls. Commonly used to stain plant root mucilage (Abeysekera & McCully, 1993)
Can be used in the quantitative determination of acid and/or sulfated algal polysaccharides in an aqueous solution, or to differentiate sulfated and non-sulfated polysaccharides in algal tissues (Ramus, 1977; Parker & Diboll, 1966).
References
Abeysekera R.M. and M. E. McCully (1993) The Epidermal Surface of the Maize Root Tip. I. Development in Normal Roots. New Phytologist, 125(2) pp. 413-429.Melissa A. Melan and Dean P. Whittier (1989) Characterization of Mucilage on the Proximal Cells of Young Gametophytes of Botrychium dissectum (Ophioglossaceae). Amer. J. Bot. 76(7): 1006-1014.Parker B.C. and A.G. Diboll (1966) Alcian Stains for Histochemical Localization of Acid and Sulfated Polysaccharides in Algae. Phycologia, 6(1), pp. 37-46.Pearse, A.G.E. (1968) Histochemistry theoretical and applied. 3rd ed. Vol. 1. Baltimore: Williams and Wilkins Co. Ramus J. (1977) Alcian Blue: A Quantitative Aqueous Assay for Algal Acid and Sulfated Polysaccharides. Journal of Phycology 13(4), pp. 345–348. DOI: 10.1111/j.1529-8817.1977.tb02939.x
