ATZ Labs - a subsidiary of Life Technologies (India) Pvt. Ltd.
Life Technologies India
PhytoTechnology Laboratories

Alcian Blue
Product ID: A1286


Description: A biological stain useful for staining pectins in plant cell walls.Synonyms: Alcian Blue 8GX, Ingrain Blue 1, C.I. 74240

Solubility Water
Physical Form Solid
Storage Temp. Room Temperature
UPC / SKU A1286
CAS NUMBER 33864-99-2
Formula Weight 1298.86
Formula C56H68Cl4CuN16S4
Synonyms Alcian Blue 8GX, Ingrain Blue 1, C.I. 74240
Storage Temp. Room Temperature
Tariff Code 3204.90.0000
Risk Info (R) 36/37/38
Safety Info (S) 36/37/39
No information available

A1286 Alcian Blue

Synonyms: Alcian Blue 8GX, Ingrain Blue 1, and C.I. 74240
CAS: 33864-99-2
Formula: C56H68Cl4CuN16S4
Mol. Weight: 1298.86
Form: Powder
Appearance: Blue or Greenish-Black to Purple
Application: Biological Stain, Phycology
Solubility: Soluble in Water
Typical Working Concentration: Varies with Application, should be determined by end user
Storage Temp: Room Temperature

Application Notes
A widely used cationic dye used to stain acid mucopolysaccharides and glycosaminoglycans. The stained tissues are blue to bluish-green in color. Particularly useful in staining pectins present in plant cell walls. Commonly used to stain plant root mucilage (Abeysekera & McCully, 1993)

Can be used in the quantitative determination of acid and/or sulfated algal polysaccharides in an aqueous solution, or to differentiate sulfated and non-sulfated polysaccharides in algal tissues (Ramus, 1977; Parker & Diboll, 1966).

Abeysekera R.M. and M. E. McCully (1993) The Epidermal Surface of the Maize Root Tip. I. Development in Normal Roots. New Phytologist, 125(2) pp. 413-429.Melissa A. Melan and Dean P. Whittier (1989) Characterization of Mucilage on the Proximal Cells of Young Gametophytes of Botrychium dissectum (Ophioglossaceae). Amer. J. Bot. 76(7): 1006-1014.Parker B.C. and A.G. Diboll (1966) Alcian Stains for Histochemical Localization of Acid and Sulfated Polysaccharides in Algae. Phycologia, 6(1), pp. 37-46.Pearse, A.G.E. (1968) Histochemistry theoretical and applied. 3rd ed. Vol. 1. Baltimore: Williams and Wilkins Co. Ramus J. (1977) Alcian Blue: A Quantitative Aqueous Assay for Algal Acid and Sulfated Polysaccharides. Journal of Phycology 13(4), pp. 345–348. DOI: 10.1111/j.1529-8817.1977.tb02939.x