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PhytoTechnology Laboratories

Arabidopsis Germination Medium
Product ID: A1375


Description: Do you have to add components to MS-media for Arabidopsis seed germination? New to PhytoTech, product A1375 is a complete formulation for Arabidopsis, containing all the components needed for seed germination. See Product Info Sheet for formulation.

This formulation contains ˝ MS-basal media with 0.5 g/L MES and 10 g/L sucrose. If you prefer 1x MS for Arabidopsis germination, we would suggest using M530 (contains M519 + 0.5 g/L MES) and add 10 g/L of sucrose.

Solubility Water
Physical Form Solid
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 12.67
Other Notes Plant Tissue Culture Tested
Storage Temp. 2-6 °C
Tariff Code 3822.00.5090
No information available

A1375 Arabidopsis Germination Medium

Synonym: Arabidopsis seed germination medium

Form: Fine to Fluffy Powder
Appearance: White to Yellow Powder
Application: Arabidopsis seed germination
Solubility: Water
Typical Working Concentration: 12.67 g/L
Storage Temp: 2-6°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Murashige and Skoog (1962). pH = 3.5-4.5
Formula (mg/L)
Ammonium Nitrate  825
 Potassium Phosphate Monobasic 85.0
 Potassium Nitrate 950
Zinc Sulfate 7H2O 4.3
 Boric Acid 3.1
 Calcium Chloride Anhydrous 166.1
Cobalt Chloride 6H2O 0.0125
MES 500
 Cupric Sulfate 5H2O 0.0125
Sucrose 10,000
Na2 EDTA 2H2O 18.63
 Ferrous Sulfate 7H2O 13.9
 Magnesium Sulfate Anhydrous 90.35
 Manganese Sulfate H2O 8.45
Sodium Molybdate 2H2O 0.125
Potassium Iodide 0.415

Application Notes
Arabidopsis thaliana (Col-0) and (Ler) possess a relatively low level of seed dormancy, and it is most often broken by stratification (2-6°C) in the dark for 2-4 days.
This formulation contains 1/2X MS (M524) with 0.5 g/L MES, and 10 g/L of sucrose and meets what the ABRC (Arabidopsis Biological Resource Center) recommends ( for Arabidopsis seed germination.
This formulation also contains enough nitrogen (20 mM in nitrate) and carbon (30 mM in sucrose) for proper seedling growth and development (Martin et al. 2002). As Arabidopsis thaliana is a model flowering plant, this type of formulation has been used often for germinating seeds (Denness et al. 2011; Stacey et al. 2006; Sorek et al. 2014).

If you prefer 1X MS for Arabidopsis germination, we would suggest using M530 (contains M519 + 0.5 g/L MES) and add 10 g/L of sucrose (Valvekens et al. 1988; Millet et al. 2010). To add Gamborg’s vitamins add 1 mL/L of G219.

Denness L, McKenna JF, Segonzac C, Wormit A, Madhou P, Bennett M, Mansfield J, Zipfel C, and Hamann T. (2011) Cell Wall Damage-Induced Lignin Biosynthesis Is Regulated by a Reactive Oxygen Species- and Jasmonic Acid-Dependent Process in Arabidopsis. Plant Physiol. Jul 2011; 156(3): 1364–1374.

Martin T, Oswald O, and Graham IA (2002) Arabidopsis Seedling Growth, Storage Lipid Mobilization, and Photosynthetic Gene Expression Are Regulated by Carbon:Nitrogen Availability. Plant Physiol. Vol. 128 (2) pg. 472-481

Stacey MG, Osawa H, Patel A, Gassmann W, and Stacey G (2006) Expression analyses of Arabidopsis oligopeptide transporters during seed germination, vegetative growth and reproduction. Planta Vol. 223(2) pg. 291- 305

Sorek N, Sorek H, Kijac A, Szemenyei HJ, Bauer S, Hématy K, Wemmer DE, and Somerville CR (2014) The Arabidopsis COBRA protein facilitates cellulose crystallization at the plasma membrane. JBC First published online 20Oct2014 doi: 10.1074/jbc.M114.607192

Valvekens D, Van Montagu M, and Van Lijsebettens M. (1988) Agrobacterium tumefaciens- mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection. PNAS 85, 5536-5540

Millet YA, Danna CH, Clay NK, Songnuan W, Simon MD, Werck-Reichhart D, and Ausubel FM (2010) Innate Immune Responses Activated in Arabidopsis Roots by Microbe-Associated Molecular Patterns. Plant Cell Vol. 22 pg. 973-990