Introduction
Description: Contains the macro- and micronutrients as described by Anderson (1978, 1980).
Anderson achieved a two-fold increase in multiplication of red raspberries using this formulation compared to MS. The optimal concentrations of growth regulators for shoot multiplication of red and black raspberries was 0.1 - 2.5 µM IBA and 4.5 - 9.0 µM 6-BA.
| Solubility |
Water |
| Physical Form |
Solid |
| Storage Temp. |
2-6 °C |
| Grams of powder to prepare 1 Liter |
1.89 |
| Other Notes |
Plant Tissue Culture Tested |
| UPC / SKU |
A267 |
| Storage Temp. |
2-6 °C |
| Tariff Code |
3822.00.5090 |
| Risk Info (R) |
8-36/37/38 |
| Safety Info (S) |
17-26-36 |
No information available
| PROD. # |
COMPONENT NAME |
mg/L |
| A300 |
Ammonium Nitrate |
400 |
| B210 |
Boric Acid |
6.2 |
| C266 |
Calcium Chloride, Anhydrous |
332.2 |
| C350 |
Cobalt Chloride Hexahydrate |
0.025 |
| C375 |
Cupric Sulfate, Pentahydrate |
0.025 |
| E410 |
EDTA, Disodium Salt |
74.5 |
| F263 |
Ferrous Sulfate, Heptahydrate |
55.7 |
| M150 |
Magnesium Sulfate, Anhydrous |
180.7 |
| M250 |
Manganese Sulfate |
16.9 |
| P840 |
Potassium Iodide |
0.3 |
| P100 |
Potassium Nitrate |
480 |
| M651 |
Sodium Molybdate (VI), Dihydrate |
0.25 |
| S515 |
Sodium Phosphate, Monobasic |
330.6 |
| Z101 |
Zinc Sulfate, Heptahydrate |
8.6 |
A267 Anderson Basal Salt Mixture
| Properties |
|---|
| Form: |
Powder |
| Appearance: |
White to Yellow Powder |
| Application: |
Plant Tissue Culture |
| Solubility: |
Water |
| Typical Working Concentration: |
1.89 g/L |
| Storage Temp: |
2-6°C |
| Storage Temp of Stock Solution: |
Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: |
Contains the macro- and micronutrients as described by Anderson (1978, 1980) pH = 3.25 – 4.25 |
| Formula (mg/L) |
|---|
Ammonium Nitrate |
400 |
| Boric Acid |
6.2 |
| Calcium Chloride, Anhydrous |
332.2 |
| Cobalt Chloride·6H2O |
0.025 |
| Cupric Sulfate·5H2O |
0.025 |
| Na2 EDTA·2H2O |
74.5 |
| Ferrous Sulfate·7H2O |
55.7 |
| Magnesium Sulfate, Anhydrous |
180.7 |
| Manganese Sulfate·H2O |
16.9 |
| Molybdic Acid (Sodium Salt)·2H2O |
0.25 |
| Potassium Iodide |
0.3 |
| Potassium Nitrate |
480 |
| Sodium Phosphate Monobasic |
330.6 |
| Zinc Sulfate·7H2O |
8.6 |
Application Notes
Plant Tissue Culture Tested Plant Species: Rhododendron, Rubus (red and black raspberry).
Anderson achieved a two-fold increase in multiplication of red raspberries using this formulation compared to MS. The optimal concentrations of growth regulators for shoot multiplication of red and black raspberries was 0.1 – 2.5 µM IBA and 4.5 – 9.0 µM BA. Anderson’s medium contains approximately ¼ strength NH4NO3 and KNO3 compared to MS.
References
Anderson, WC. 1978. Tissue culture propagation of Rhododendrons. In Vitro 14: 334. Anderson, WC. 1980. Tissue culture propagation of red and black raspberries ,Rubus idaeus and R. occidentalis . Acta Hort. 112: 13-20.
