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PhytoTechnology Laboratories

Broadleaf Tree Basal Medium
Product ID: B1396


Description: Contains 0.2 mg/L 6-Benzylaminopurine (BA, product B800).
Contains the macro- and micronutrients, vitamins, and supplements required to culture orchids.

Solubility Water
Physical Form Solid
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 1.21
Other Notes Plant Tissue Culture Tested
UPC / SKU B1396
Storage Temp. 2-6 °C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
A300 Ammonium Nitrate 82.5
A305 Ammonium Sulfate 120
B210 Boric Acid 1.55
C266 Calcium Chloride, Anhydrous 16.6
C180 Calcium Nitrate 222
C350 Cobalt Chloride Hexahydrate 0.063
C375 Cupric Sulfate, Pentahydrate 0.063
E410 EDTA, Disodium Salt
F263 Ferrous Sulfate, Heptahydrate 6.95
M150 Magnesium Sulfate, Anhydrous 90
M250 Manganese Sulfate 4.225
P840 Potassium Iodide 0.038
P100 Potassium Nitrate 95
P846 Potassium Phosphate, Monobasic, Anhydrous 85
P854 Potassium Sulfate, Anhydrous 430
M651 Sodium Molybdate (VI), Dihydrate 0.063
Z101 Zinc Sulfate, Heptahydrate 2.15
I703 myo-Inositol 50
N765 Nicotinic Acid 0.25

B1396 Broadleaf Tree Basal Medium

Form: Powder
Appearance: White to Yellow
Application: Plant Tissue Culture
Solubility: Soluble in Water
Typical Working Concentration: 1.21 g/L
Storage Temp: 2-6°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Formula (mg/L)
Ammonium Nitrate 82.5
 Manganese Sulfate, Monohydrate  4.225
 Ammonium Sulfate  120
 Molybdic Acid, Disodium, Dihydrate  0.063
 Boric Acid  1.55
 Potassium Iodide 0.038
 Calcium Chloride 16.6
Potassium Nitrate  95
 Calcium Nitrate 222
Potassium Phosphate, Monobasic 85
 Cobalt Chloride, Hexahydrate 0.005
 Potassium Sulfate  430
 Cupric Sulfate, Pentahydrate 0.063
 Zinc Sulfate, Heptahydrate 2.15
 EDTA, Disodium, Dihydrate 9.325
 myo-Inositol  50
Ferrous Sulfate, Heptahydrate 6.95
Nicotinic Acid 0.25
 Magnesium Sulfate, Anhydrous  90

Application Notes
Plant tissue culture tested. Plant Species: oak (Quereus), linden (Tilia), and related broadleaf trees. Contains the micro- and macro-nutrients and vitamins described by Chalupa (1984).

Chalupa, 1984. Biologia Plantarum. 26(5):374-377.